OAZ Antibody (E-6): sc-393904

OAZ Antibody (E-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects OAZ in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). OAZ (E-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. OAZ, also known as Olf-1/EBF associated zinc finger or Roaz, is a 30-zinc finger DNA-binding protein that plays a crucial role in the regulation of gene expression in response to bone morphogenic protein (BMP) signaling. BMPs are vital components of the transforming growth factor-beta (TGFβ) superfamily, influencing key developmental processes such as left-right asymmetry, neurogenesis, organogenesis, and skeletal development. The interaction of OAZ with BMP-activated Smads—specifically Smad1, 5, and 8—facilitates binding to BMP response elements, leading to transcriptional activation of target genes. The structural integrity of OAZ, characterized by unique zinc finger clusters, is essential for dual functionality; OAZ not only mediates BMP signaling but also acts as a transcriptional partner for Olf-1/EBF in the development of the olfactory epithelium and lymphocytes. This multifaceted role underscores OAZ′s importance in developmental biology and signal transduction, making anti-OAZ antibody (E-6) a valuable tool for researchers studying these critical pathways.

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OAZ Antibody (E-6) References:

1. Zinc finger peptides for the regulation of gene expression.  |  Klug, A. 1999. J Mol Biol. 293: 215-8. PMID: 10529348
2. Regulation of Smad activity.  |  Wrana, JL. 2000. Cell. 100: 189-92. PMID: 10660041
3. OAZ uses distinct DNA- and protein-binding zinc fingers in separate BMP-Smad and Olf signaling pathways.  |  Hata, A., et al. 2000. Cell. 100: 229-40. PMID: 10660046
4. OAZ regulates bone morphogenetic protein signaling through Smad6 activation.  |  Ku, M., et al. 2006. J Biol Chem. 281: 5277-87. PMID: 16373339
5. Zfp423/OAZ participates in a developmental switch during olfactory neurogenesis.  |  Cheng, LE. and Reed, RR. 2007. Neuron. 54: 547-57. PMID: 17521568
6. OAZ-t/OAZ3 is essential for rigid connection of sperm tails to heads in mouse.  |  Tokuhiro, K., et al. 2009. PLoS Genet. 5: e1000712. PMID: 19893612
7. Zfp423/OAZ mutation reveals the importance of Olf/EBF transcription activity in olfactory neuronal maturation.  |  Roby, YA., et al. 2012. J Neurosci. 32: 13679-88a. PMID: 23035080
8. Effects of age and oligoasthenozoospermia on telomeres of sperm and blood cells.  |  Balmori, C., et al. 2022. Reprod Biomed Online. 44: 1090-1100. PMID: 35397997
9. Bone morphogenetic proteins: multifunctional regulators of vertebrate development.  |  Hogan, BL. 1996. Genes Dev. 10: 1580-94. PMID: 8682290
10. Cloning and functional characterization of Roaz, a zinc finger protein that interacts with O/E-1 to regulate gene expression: implications for olfactory neuronal development.  |  Tsai, RY. and Reed, RR. 1997. J Neurosci. 17: 4159-69. PMID: 9151733
11. Specific activation of Smad1 signaling pathways by the BMP7 type I receptor, ALK2.  |  Macías-Silva, M., et al. 1998. J Biol Chem. 273: 25628-36. PMID: 9748228
12. Identification of DNA recognition sequences and protein interaction domains of the multiple-Zn-finger protein Roaz.  |  Tsai, RY. and Reed, RR. 1998. Mol Cell Biol. 18: 6447-56. PMID: 9774661

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-OAZ Antibody (E-6) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-OAZ Antibody (E-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

OAZ Antibody (E-6) Specifications

Alternate Product Name: OAZ

Product Clone Name: E-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 35-58

Epitope Target: OAZ

Epitope Species: human

Product Reactivity/Detection: OAZ

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: avian

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for OAZ Antibody (E-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent

The preferred OAZ antibody is OAZ Antibody (B-7): sc-514748.