Nup93 Antibody (E-8) recommended for detection of Nup93 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Nup93 Antibody (E-8): sc-374399

Nup93 Antibody (E-8) is rated 4.9 out of 5 by 8.
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Datasheets

    • Anti-Nup93 Antibody (E-8) is a mouse monoclonal IgG1 (kappa light chain) Nup93 antibody provided at 200 µg/ml
    • raised against amino acids 1-300 mapping at the N-terminus of Nup93 of human origin
    • Anti-Nup93 Antibody (E-8) is recommended for detection of Nup93 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Nup93 Antibody (E-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Nup93 (E-8): sc-374399.

Can Nup93 (E-8): sc-374399 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jerojero
Thank you for your question. Yes, Nup93 (E-8): sc-374399 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24

この抗体を注文予定のものですが、すごくきれいに免疫染色でも染まっていますが、 パラフィン切片での染色(蛍光抗体法)を検討中なのですが、抗原賦活の方法や、具体的な染色手順を ご教示いただけませんでしょうか。よろしくお願いいたいします。

Asked by: shiroko
Thank you for your question. For detailed protocol information, please refer to our online protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining お問い合わせを頂きまして、誠にありがとうございました。 プロトコルの参考については、こちらをご覧ください: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support 5
Date published: 2017-02-10
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Rated 5 out of 5 by from Nice antibody against Nup93 This antibody at 1:200 dilution can detect a very specific band.
Date published: 2017-11-21
Rated 4 out of 5 by from nice staining in IF assay I got the free trial and it showed good staining in the cells.
Date published: 2017-10-12
Rated 5 out of 5 by from Excellent for WB it works excellent for WB in our lab, will keep usinig it
Date published: 2017-09-18
Rated 5 out of 5 by from Good for multiple applications Strong antibody performance in Western Blot, IF and IHC!
Date published: 2017-01-17
Rated 5 out of 5 by from Great production! The antibody works well in western blot and immunofluorescence.
Date published: 2016-11-02
Rated 5 out of 5 by from Antibody is working well no problems Antibody is working well no problems.
Date published: 2015-08-26
Rated 5 out of 5 by from Produced superb immunoperoxidase nuclear Produced superb immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human esophagus tissue. -SCBT QC
Date published: 2015-04-28
Rated 5 out of 5 by from Good specificity in WB in 293T Good specificity in WB in 293T, T24, LADMAQC and MTE1D whole cell lysates. -SCBT QC
Date published: 2015-04-16
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