NuMA Antibody (F-11): sc-365532

NuMA Antibody (F-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects NuMA protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). NuMA (F-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NuMA protein, also known as Nuclear Mitotic Apparatus Protein 1, plays a critical role in organizing and stabilizing spindle poles during mitosis, which is essential for accurate chromosome segregation. Mapping to chromosome 11q13, NuMA gene encodes a noncentrosomal protein that binds to microtubules through its carboxyl terminal region, facilitating bipolar spindle formation by aggregating microtubules and interacting with motor proteins. This function is vital for maintaining genomic stability, as errors in spindle assembly can lead to aneuploidy and contribute to tumorigenesis. NuMA protein is predominantly localized to the spindle apparatus during mitosis, yet also appears in the nucleus and cytoplasm of post-mitotic neurons, indicating diverse roles in different cell types. Elevated NuMA expression levels have been associated with various cancers, particularly colorectal carcinoma, highlighting potential value as a biomarker for cancer diagnosis and prognosis.

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NuMA Antibody (F-11) References:

1. Nuclear mitotic apparatus protein (NuMA) in benign and malignant diseases.  |  Hasholzner, U., et al. 1999. Anticancer Res. 19: 2415-20. PMID: 10470168
2. The nuclear/mitotic apparatus protein NuMA is a component of the somatodendritic microtubule arrays of the neuron.  |  Ferhat, L., et al. 1998. J Neurocytol. 27: 887-99. PMID: 10659681
3. NuMA: a nuclear protein involved in mitotic centrosome function.  |  Zeng, C. 2000. Microsc Res Tech. 49: 467-77. PMID: 10842374
4. Chromosome movement in mitosis requires microtubule anchorage at spindle poles.  |  Gordon, MB., et al. 2001. J Cell Biol. 152: 425-34. PMID: 11157972
5. NuMA influences higher order chromatin organization in human mammary epithelium.  |  Abad, PC., et al. 2007. Mol Biol Cell. 18: 348-61. PMID: 17108325
6. NuMA overexpression in epithelial ovarian cancer.  |  Brüning-Richardson, A., et al. 2012. PLoS One. 7: e38945. PMID: 22719996
7. NuMA recruits dynein activity to microtubule minus-ends at mitosis.  |  Hueschen, CL., et al. 2017. Elife. 6: PMID: 29185983
8. Dynein-Dynactin-NuMA clusters generate cortical spindle-pulling forces as a multi-arm ensemble.  |  Okumura, M., et al. 2018. Elife. 7: PMID: 29848445
9. NuMA assemblies organize microtubule asters to establish spindle bipolarity in acentrosomal human cells.  |  Chinen, T., et al. 2020. EMBO J. 39: e102378. PMID: 31782546
10. Human-specific nuclear protein that associates with the polar region of the mitotic apparatus: distribution in a human/hamster hybrid cell.  |  Lydersen, BK. and Pettijohn, DE. 1980. Cell. 22: 489-99. PMID: 7004645
11. NuMA, a nuclear protein involved in mitosis and nuclear reformation.  |  Compton, DA. and Cleveland, DW. 1994. Curr Opin Cell Biol. 6: 343-6. PMID: 7917323
12. Assignment of the nuclear mitotic apparatus protein NuMA gene to human chromosome 11q13.  |  Sparks, CA., et al. 1993. Genomics. 17: 222-4. PMID: 8406455

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NuMA Antibody (F-11) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-NuMA Antibody (F-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NuMA Antibody (F-11) Specifications

Alternate Product Name: NUMA1

Product Clone Name: F-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1816-2115

Epitope Target: NuMA

Epitope Species: human

Product Reactivity/Detection: NuMA

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for NuMA Antibody (F-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin