NuMA Activators
NuMA, or Nuclear Mitotic Apparatus protein, stands as a pivotal player in orchestrating mitotic spindle assembly and maintenance, thereby ensuring faithful chromosome segregation during cell division. Functionally, NuMA serves as a scaffold protein localized at the nucleus and the spindle poles, where it coordinates the dynamic interactions between microtubules, motor proteins, and other spindle-associated factors critical for mitotic progression. NuMA's central role lies in its ability to tether and stabilize microtubules emanating from the spindle poles, forming robust bipolar spindles essential for proper chromosome alignment and segregation. Additionally, NuMA contributes to the establishment of cell polarity and spindle orientation during mitosis, facilitating asymmetric cell division and tissue organization in multicellular organisms.
The activation of NuMA is tightly regulated by a complex interplay of post-translational modifications, protein-protein interactions, and cellular signaling pathways governing mitotic progression. Phosphorylation emerges as a key mechanism driving NuMA activation, with several mitotic kinases, including Aurora-A, Plk1, and Cdk1, phosphorylating distinct residues within NuMA to modulate its localization, activity, and interaction with microtubules. Furthermore, NuMA undergoes dynamic changes in its subcellular localization throughout the cell cycle, translocating from the nucleus to the spindle poles during mitosis, where it exerts its essential functions in spindle assembly and organization. Beyond direct phosphorylation events, NuMA activation is intricately linked to the activity of other mitotic regulators and signaling pathways, such as the Ran-GTP gradient and the Astrin-SKAP complex, which collectively contribute to the spatial and temporal regulation of NuMA function during mitotic progression. Overall, elucidating the multifaceted mechanisms underlying NuMA activation provides invaluable insights into the fundamental processes driving accurate chromosome segregation and cell division.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $41.00 $132.00 $214.00 $500.00 $948.00 | 119 | |
As a diacylglycerol analog, PMA activates Protein Kinase C (PKC), which can phosphorylate targets that might be involved in NuMA activation during mitosis. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $291.00 $530.00 $1800.00 | 78 | |
It is a potent inhibitor of protein phosphatases 1 and 2A, preventing dephosphorylation, thereby indirectly maintaining NuMA in a phosphorylated state. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A | 10 µg 100 µg | $163.00 $800.00 | 59 | |
Similar to okadaic acid, this compound inhibits phosphatases, leading to an increase in phosphorylation levels of proteins, which may include those involved in activating NuMA. | ||||||
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $78.00 $153.00 $740.00 $1413.00 $2091.00 | 73 | |
By elevating cAMP levels, forskolin activates PKA, which could potentially phosphorylate intermediary proteins that regulate NuMA activity. | ||||||
8-Bromo-cAMP | 76939-46-3 | sc-201564 sc-201564A | 10 mg 50 mg | $126.00 $328.00 | 30 | |
This cAMP analog can activate PKA, mimicking the effects of forskolin, and potentially influencing NuMA activation through phosphorylation. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $99.00 $259.00 | 36 | |
Known to activate stress-activated protein kinases, anisomycin might influence NuMA function indirectly through stress pathways. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $78.00 $270.00 | 80 | |
As a calcium ionophore, ionomycin raises intracellular calcium levels, which can activate calcium-dependent kinases that might phosphorylate NuMA-associated proteins. | ||||||
5-Iodotubercidin | 24386-93-4 | sc-3531 sc-3531A | 1 mg 5 mg | $153.00 $464.00 | 20 | |
A potent adenosine kinase inhibitor, by increasing adenosine levels, can indirectly influence kinase activity relevant to NuMA function. | ||||||
Angiotensin II, Human | 4474-91-3 | sc-363643 sc-363643A sc-363643B sc-363643C | 1 mg 5 mg 25 mg 100 mg | $51.00 $100.00 $310.00 $690.00 | 3 | |
This peptide hormone can activate various kinases through its receptor-mediated signaling, potentially affecting NuMA phosphorylation indirectly. | ||||||
Insulin | 11061-68-0 | sc-29062 sc-29062A sc-29062B | 100 mg 1 g 10 g | $156.00 $1248.00 $12508.00 | 82 | |
Through the PI3K/Akt pathway, insulin can initiate a signaling cascade that culminates in the phosphorylation of proteins potentially involved in NuMA regulation. | ||||||