NuMA Antibody (A73-B/D12): sc-56325
- NuMA Antibody (A73-B/D12) is a mouse monoclonal IgM, cited in 3 publications, provided at 50 µg/500 µl
- raised against Ls 174T cell line of human origin
- recommended for detection of NuMA (found in the nucleus during interphase) of human origin by WB, IP, IF and IHC(P)
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for NuMA Antibody (A73-B/D12). This work is in progress.
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NuMA Antibody (A73-B/D12) is a mouse monoclonal IgM antibody that detects NuMA in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. NuMA, or nuclear mitotic apparatus protein, plays a critical role in the organization and stabilization of spindle poles during mitosis, which is essential for accurate chromosome segregation and cell division. NuMA (A73-B/D12) antibody recognizes a protein primarily located in the nucleus during interphase, but upon mitotic entry, NuMA localizes to the spindle apparatus, where NuMA forms aggregates that interact with microtubules and motor proteins. This interaction is vital as NuMA helps to organize microtubules into a bipolar spindle, ensuring that chromosomes are evenly distributed to daughter cells. In post-mitotic neurons, NuMA is found both in the nucleus and cytoplasm, indicating diverse roles beyond mitosis. Elevated expression levels of NuMA have been associated with various cancers, particularly colorectal carcinoma, highlighting NuMA′s potential as a biomarker for cancer diagnosis and prognosis. Anti-NuMA antibody (A73-B/D12) versatility in various applications makes NuMA research invaluable for studying cell division, cancer biology, and mitotic apparatus dynamics.
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NuMA Antibody (A73-B/D12) References:
- On the inscrutable role of Inscuteable: structural basis and functional implications for the competitive binding of NuMA and Inscuteable to LGN. | Mapelli, M. and Gonzalez, C. 2012. Open Biol. 2: 120102. PMID: 22977735
- NuMA phosphorylation dictates dynein-dependent spindle positioning. | Kotak, S. and Gönczy, P. 2014. Cell Cycle. 13: 177-8. PMID: 24241204
- Identification and characterization of novel NuMA isoforms. | Wu, J., et al. 2014. Biochem Biophys Res Commun. 454: 387-92. PMID: 25451259
- NuMA Phosphorylation by Aurora-A Orchestrates Spindle Orientation. | Gallini, S., et al. 2016. Curr Biol. 26: 458-69. PMID: 26832443
- NuMA interaction with chromatin is vital for proper chromosome decondensation at the mitotic exit. | Rajeevan, A., et al. 2020. Mol Biol Cell. 31: 2437-2451. PMID: 32845810
- The Nuclear Mitotic Apparatus (NuMA) Protein: A Key Player for Nuclear Formation, Spindle Assembly, and Spindle Positioning. | Kiyomitsu, T. and Boerner, S. 2021. Front Cell Dev Biol. 9: 653801. PMID: 33869212
- NuMA regulates mitotic spindle assembly, structural dynamics and function via phase separation. | Sun, M., et al. 2021. Nat Commun. 12: 7157. PMID: 34887424
- NuMA forms condensates through phase separation to drive spindle pole assembly. | Ma, H., et al. 2022. J Mol Cell Biol. 14: PMID: 34958389
- Membrane compartmentalization of Ect2/Cyk4/Mklp1 and NuMA/dynein regulates cleavage furrow formation. | Sana, S., et al. 2022. J Cell Biol. 221: PMID: 36197340
- NuMA deficiency causes micronuclei via checkpoint-insensitive k-fiber minus-end detachment from mitotic spindle poles. | van Toorn, M., et al. 2023. Curr Biol. 33: 572-580.e2. PMID: 36626904
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NuMA Antibody (A73-B/D12) | sc-56325 | 50 µg/500 µl | $322.00 |