NUDT21 Antibody (2203C3): sc-81109

NUDT21 Antibody (2203C3) is a mouse monoclonal IgG1 antibody that detects NUDT21 in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), and flow cytometry (FCM). Anti-NUDT21 antibody (2203C3) is available in a non-conjugated form, providing flexibility for various experimental needs. NUDT21, also known as CPSF5 (cleavage and polyadenylation specificity factor subunit 5) or CFIm25 (cleavage and polyadenylation specificity factor 25 kDa subunit), is a crucial member of the Nudix hydrolase family, characterized by the presence of a NUDIX domain that facilitates the hydrolysis of nucleoside diphosphate derivatives. NUDT21 is primarily located in the paraspeckles of the cell nucleus, where NUDT21 plays a vital role in gene expression regulation by participating in pre-mRNA cleavage and polyadenylation. NUDT21 localization to paraspeckles is significant as these structures are involved in RNA storage and processing, thereby influencing mRNA stability and transport. Additionally, NUDT21 forms a heterodimer with CPSF6 or CPSF7, contributing to the CFIm (mammalian Cleavage Factor I) complex, which is essential for proper pre-mRNA processing. NUDT21 functionality in this complex underscores NUDT21′s importance in post-transcriptional regulation, making NUDT21 a valuable target for research in gene expression and RNA processing mechanisms.

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NUDT21 Antibody (2203C3) References:

1. An Arabidopsis Fip1 homolog interacts with RNA and provides conceptual links with a number of other polyadenylation factor subunits.  |  Forbes, KP., et al. 2006. J Biol Chem. 281: 176-86. PMID: 16282318
2. Knock-down of 25 kDa subunit of cleavage factor Im in Hela cells alters alternative polyadenylation within 3'-UTRs.  |  Kubo, T., et al. 2006. Nucleic Acids Res. 34: 6264-71. PMID: 17098938
3. Multiple histone deacetylases and the CREB-binding protein regulate pre-mRNA 3'-end processing.  |  Shimazu, T., et al. 2007. J Biol Chem. 282: 4470-4478. PMID: 17172643
4. Structure and function of the E. coli dihydroneopterin triphosphate pyrophosphatase: a Nudix enzyme involved in folate biosynthesis.  |  Gabelli, SB., et al. 2007. Structure. 15: 1014-22. PMID: 17698004
5. Overexpression, crystallization and preliminary X-ray crystallographic analysis of Nudix hydrolase Orf141 from Escherichia coli K-1.  |  Jung, J., et al. 2007. Acta Crystallogr Sect F Struct Biol Cryst Commun. 63: 812-5. PMID: 17768363
6. Pre-messenger RNA cleavage factor I (CFIm): potential role in alternative polyadenylation during spermatogenesis.  |  Sartini, BL., et al. 2008. Biol Reprod. 78: 472-82. PMID: 18032416
7. Nudt21 Controls Cell Fate by Connecting Alternative Polyadenylation to Chromatin Signaling.  |  Brumbaugh, J., et al. 2018. Cell. 172: 106-120.e21. PMID: 29249356
8. NUDT21 limits CD19 levels through alternative mRNA polyadenylation in B cell acute lymphoblastic leukemia.  |  Witkowski, MT., et al. 2022. Nat Immunol. 23: 1424-1432. PMID: 36138187
9. Relationship between NUDT21 mediated alternative polyadenylation process and tumor.  |  Xiao, S., et al. 2023. Front Oncol. 13: 1052012. PMID: 36816917
10. Alternative polyadenylation reprogramming of MORC2 induced by NUDT21 loss promotes KIRC carcinogenesis.  |  Tan, Y., et al. 2023. JCI Insight. 8: PMID: 37737260
11. LINC00921 reduces lung cancer radiosensitivity by destabilizing NUDT21 and driving aberrant MED23 alternative polyadenylation.  |  Zhang, N., et al. 2023. Cell Rep. 42: 113479. PMID: 37999979
12. TRIM65 deficiency alleviates renal fibrosis through NUDT21-mediated alternative polyadenylation.  |  Wei, S., et al. 2024. Cell Death Differ. 31: 1422-1438. PMID: 38951701

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NUDT21 Antibody (2203C3) has 23 citations in a variety of scientific publications.

Primary antibodies like Anti-NUDT21 Antibody (2203C3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NUDT21 Antibody (2203C3) Specifications

Alternate Product Name: NUDT21

Product Clone Name: 2203C3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids:

Epitope Target: NUDT21

Epitope Species: human

Product Reactivity/Detection: NUDT21

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, FCM

Vialing Information: Each vial contains 100 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 1.0% stabilizer protein

Research Citations: 23 citations in various scientific publications

Available Conjugates/Forms for NUDT21 Antibody (2203C3): N/A

The preferred NUDT21 antibody is NUDT21 Antibody (F-5): sc-515766.