NT5C3 Antibody (E-8): sc-390782
- NT5C3 Antibody (E-8) is a mouse monoclonal IgG2b κ NT5C3 antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 94-202 mapping within an internal region of NT5C3L of human origin
- NT5C3 Antibody (E-8) is recommended for detection of NT5C3L of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-NT5C3 Antibody (E-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for NT5C3 Antibody (E-8) for WB applications. These reagents are now offered in bundles with NT5C3 Antibody (E-8) (see ordering information below).
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NT5C3 Antibody (E-8) is a mouse monoclonal IgG2b kappa light chain antibody that detects NT5C3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NT5C3 (E-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NT5C3, also known as 5′-nucleotidase, cytosolic III, P5N1, or UMPH1, is a crucial enzyme that plays a significant role in nucleotide metabolism by catalyzing the hydrolysis of nucleotides into nucleosides and phosphates. This enzymatic activity is vital for maintaining nucleotide homeostasis within cells, which is essential for various cellular processes, including DNA and RNA synthesis, energy metabolism, and signal transduction. NT5C3 is predominantly expressed in lymphocytes and reticulocytes, and exists as multiple alternatively spliced isoforms that localize to either the cytoplasm or the endoplasmic reticulum. NT5C3 functions as a monomer and exhibits both nucleotidase and phosphotransferase activities, effectively converting 5′-ribonucleotides to ribonucleosides and free phosphates. Defects in the gene encoding NT5C3 can lead to P5N deficiency, an autosomal recessive disorder associated with hemolytic anemia, learning difficulties, and increased susceptibility to lead poisoning, highlighting NT5C3′s critical role in cellular health and function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
NT5C3 Antibody (E-8) References:
- Human erythrocyte pyrimidine 5-nucleotidase, PN-I, is identical to p36, a protein associated to lupus inclusion formation in response to alpha-interferon. | Amici, A., et al. 2000. Blood. 96: 1596-8. PMID: 10942414
- Pyrimidine 5' nucleotidase deficiency. | Rees, DC., et al. 2003. Br J Haematol. 120: 375-83. PMID: 12580951
- Molecular characterization of Turkish patients with pyrimidine 5' nucleotidase-I deficiency. | Balta, G., et al. 2003. Blood. 102: 1900-3. PMID: 12714505
- Molecular characterization of six unrelated Italian patients affected by pyrimidine 5'-nucleotidase deficiency. | Bianchi, P., et al. 2003. Br J Haematol. 122: 847-51. PMID: 12930399
- Functional analysis of pyrimidine 5'-nucleotidase mutants causing nonspherocytic hemolytic anemia. | Chiarelli, LR., et al. 2005. Blood. 105: 3340-5. PMID: 15604219
- Overexpression of hUMP1/POMP proteasome accessory protein enhances proteasome-mediated antioxidant defence. | Chondrogianni, N. and Gonos, ES. 2007. Exp Gerontol. 42: 899-903. PMID: 17349762
- Homogeneous pyrimidine nucleotidase from human erythrocytes: enzymic and molecular properties. | Amici, A., et al. 1994. Biochem J. 304 (Pt 3): 987-92. PMID: 7818506
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NT5C3 Antibody (E-8) | sc-390782 | 200 µg/ml | $322.00 | |||
NT5C3 Antibody (E-8): m-IgGκ BP-HRP Bundle | sc-523680 | 200 µg Ab, 40 µg BP | $361.00 | |||
NT5C3 Antibody (E-8): m-IgG2b BP-HRP Bundle | sc-549133 | 200 µg Ab; 10 µg BP | $361.00 | |||
NT5C3 Antibody (E-8) AC | sc-390782 AC | 500 µg/ml, 25% agarose | $424.00 | |||
NT5C3 Antibody (E-8) HRP | sc-390782 HRP | 200 µg/ml | $322.00 | |||
NT5C3 Antibody (E-8) FITC | sc-390782 FITC | 200 µg/ml | $336.00 | |||
NT5C3 Antibody (E-8) PE | sc-390782 PE | 200 µg/ml | $349.00 | |||
NT5C3 Antibody (E-8) Alexa Fluor® 488 | sc-390782 AF488 | 200 µg/ml | $364.00 | |||
NT5C3 Antibody (E-8) Alexa Fluor® 546 | sc-390782 AF546 | 200 µg/ml | $364.00 | |||
NT5C3 Antibody (E-8) Alexa Fluor® 594 | sc-390782 AF594 | 200 µg/ml | $364.00 | |||
NT5C3 Antibody (E-8) Alexa Fluor® 647 | sc-390782 AF647 | 200 µg/ml | $364.00 | |||
NT5C3 Antibody (E-8) Alexa Fluor® 680 | sc-390782 AF680 | 200 µg/ml | $364.00 | |||
NT5C3 Antibody (E-8) Alexa Fluor® 790 | sc-390782 AF790 | 200 µg/ml | $364.00 |