NSDHL Antibody (D-11): sc-390871

NSDHL Antibody (D-11) is a mouse monoclonal IgG2b kappa light chain antibody that detects NSDHL protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-NSDHL antibody (D-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NSDHL protein, also known as NAD(P) dependent steroid dehydrogenase-like, H105E3, XAP104, or SDR31E1, is a 373 amino acid single-pass membrane protein predominantly expressed in brain, heart, liver, lung, kidney, skin, and placenta. NSDHL protein localizes to endoplasmic reticulum and plays a significant role in demethylation of sterol precursors during later stages of cholesterol synthesis. NSDHL (D-11) antibody has been linked to congenital hemidysplasia with ichthyosiform erythroderma and limb defects (CHILD), an X-linked dominant disorder resulting from defects in NSDHL gene. This condition is characterized by severe lipid metabolism issues and defective cholesterol biosynthesis, often leading to male lethality, highlighting NSDHL protein′s critical role in maintaining proper metabolic function and development.

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NSDHL Antibody (D-11) References:

1. Localization of mammalian NAD(P)H steroid dehydrogenase-like protein on lipid droplets.  |  Ohashi, M., et al. 2003. J Biol Chem. 278: 36819-29. PMID: 12837764
2. Left-sided CHILD syndrome caused by a nonsense mutation in the NSDHL gene.  |  Hummel, M., et al. 2003. Am J Med Genet A. 122A: 246-51. PMID: 12966526
3. NSDHL, an enzyme involved in cholesterol biosynthesis, traffics through the Golgi and accumulates on ER membranes and on the surface of lipid droplets.  |  Caldas, H. and Herman, GE. 2003. Hum Mol Genet. 12: 2981-91. PMID: 14506130
4. A unique point mutation in the NSDHL gene in a Japanese patient with CHILD syndrome.  |  Murata, K., et al. 2003. J Dermatol Sci. 33: 67-9. PMID: 14527740
5. A novel somatic mutation of the 3beta-hydroxysteroid dehydrogenase gene in sporadic cutaneous verruciform xanthoma.  |  Mehra, S., et al. 2005. Arch Dermatol. 141: 1263-7. PMID: 16230564
6. CHILD syndrome in 3 generations: the importance of mild or minimal skin lesions.  |  Bittar, M., et al. 2006. Arch Dermatol. 142: 348-51. PMID: 16549711
7. Functional analysis of cholesterol biosynthesis by RNA interference.  |  Guggenberger, C., et al. 2007. J Steroid Biochem Mol Biol. 104: 105-9. PMID: 17498944
8. A novel silent mutation in the NSDHL gene causing CHILD syndrome as a result of aberrant splicing.  |  Saito, M. and Ishiko, A. 2008. Br J Dermatol. 159: 1204-6. PMID: 18764845
9. Developmental expression pattern of the cholesterogenic enzyme NSDHL and negative selection of NSDHL-deficient cells in the heterozygous Bpa(1H)/+ mouse.  |  Cunningham, D., et al. 2009. Mol Genet Metab. 98: 356-66. PMID: 19631568

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NSDHL Antibody (D-11) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-NSDHL Antibody (D-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NSDHL Antibody (D-11) Specifications

Alternate Product Name: NSDHL

Product Clone Name: D-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-195

Epitope Target: NSDHL

Epitope Species: human

Product Reactivity/Detection: NSDHL

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for NSDHL Antibody (D-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin