NSD2 Antibody (G-12): sc-365627

NSD2 Antibody (G-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects NSD2 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NSD2 (G-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The WHSC1 gene encodes NSD2, which plays a crucial role in histone methylation, specifically trimethylating histone H3 at lysine 36, a modification essential for regulating gene expression and maintaining chromatin structure. This function is particularly important in development and cellular differentiation, as aberrations in histone methylation can lead to various diseases, including cancer. Mutations or deletions in the WHSC1 gene are associated with Wolf-Hirschhorn syndrome, a genetic disorder characterized by developmental issues, including cognitive impairment and physical malformations. NSD2 is expressed widely in rapidly growing embryonic tissues, aligning with affected organs in patients with Wolf-Hirschhorn syndrome. Alternative splicing of the WHSC1 gene results in multiple transcript variants encoding different NSD2 isoforms, highlighting this protein′s complexity and significance in cellular processes.

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NSD2 Antibody (G-12) References:

1. First known microdeletion within the Wolf-Hirschhorn syndrome critical region refines genotype-phenotype correlation.  |  Rauch, A., et al. 2001. Am J Med Genet. 99: 338-42. PMID: 11252005
2. A subset of multiple myeloma harboring the t(4;14)(p16;q32) translocation lacks FGFR3 expression but maintains an IGH/MMSET fusion transcript.  |  Santra, M., et al. 2003. Blood. 101: 2374-6. PMID: 12433679
3. [Genetic determination of Wolf-Hirschhorn syndrome ].  |  Gutmajster, E. and Rokicka, A. 2002. Wiad Lek. 55: 706-10. PMID: 12715353
4. Evaluation of NSD2 and NSD3 in overgrowth syndromes.  |  Douglas, J., et al. 2005. Eur J Hum Genet. 13: 150-3. PMID: 15483650
5. Overexpression of transcripts originating from the MMSET locus characterizes all t(4;14)(p16;q32)-positive multiple myeloma patients.  |  Keats, JJ., et al. 2005. Blood. 105: 4060-9. PMID: 15677557
6. The etiology of Wolf-Hirschhorn syndrome.  |  Bergemann, AD., et al. 2005. Trends Genet. 21: 188-95. PMID: 15734578
7. Identification of ID-1 as a potential target gene of MMSET in multiple myeloma.  |  Hudlebusch, HR., et al. 2005. Br J Haematol. 130: 700-8. PMID: 16115125
8. Transcription repression activity is associated with the type I isoform of the MMSET gene involved in t(4;14) in multiple myeloma.  |  Todoerti, K., et al. 2005. Br J Haematol. 131: 214-8. PMID: 16197452
9. Characterization of a novel WHSC1-associated SET domain protein with H3K4 and H3K27 methyltransferase activity.  |  Kim, SM., et al. 2006. Biochem Biophys Res Commun. 345: 318-23. PMID: 16682010
10. NSD2 dimethylation at H3K36 promotes lung adenocarcinoma pathogenesis.  |  Sengupta, D., et al. 2021. Mol Cell. 81: 4481-4492.e9. PMID: 34555356
11. Discovery of a Potent and Selective Targeted NSD2 Degrader for the Reduction of H3K36me2.  |  Hanley, RP., et al. 2023. J Am Chem Soc. 145: 8176-8188. PMID: 36976643
12. Recruitment of FBXO22 for Targeted Degradation of NSD2.  |  Nie, DY., et al. 2023. bioRxiv.. PMID: 37961297

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NSD2 Antibody (G-12) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-NSD2 Antibody (G-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NSD2 Antibody (G-12) Specifications

Alternate Product Name: NSD2

Product Clone Name: G-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-120

Epitope Target: NSD2

Epitope Species: human

Product Reactivity/Detection: NSD2

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for NSD2 Antibody (G-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, B: Biotin, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin