NRAMP 2 Antibody (G-5): sc-166884

NRAMP 2 Antibody (G-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects NRAMP 2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-NRAMP 2 antibody (G-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The natural resistance associated macrophage proteins (NRAMPs) are integral membrane proteins that play crucial roles in metal ion transport, with NRAMP 2 functioning as a divalent cation transporter. NRAMP 2 is predominantly expressed in the duodenum, where expression is regulated by dietary iron levels at the brush border, highlighting NRAMP 2′s importance in iron homeostasis and absorption. The gene encoding human NRAMP 2 is located on chromosome 12q13.12, and NRAMP 2 expression is vital for maintaining iron balance in the body. Mutations in NRAMP 2 gene in animal models have been linked to severe anemia, underscoring NRAMP 2′s critical role in iron metabolism and potential implications in various hematological disorders.

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NRAMP 2 Antibody (G-5) References:

1. The iron transport protein NRAMP2 is an integral membrane glycoprotein that colocalizes with transferrin in recycling endosomes.  |  Gruenheid, S., et al. 1999. J Exp Med. 189: 831-41. PMID: 10049947
2. Cellular and subcellular localization of the Nramp2 iron transporter in the intestinal brush border and regulation by dietary iron.  |  Canonne-Hergaux, F., et al. 1999. Blood. 93: 4406-17. PMID: 10361139
3. Natural-resistance-associated macrophage protein 1 is an H+/bivalent cation antiporter.  |  Goswami, T., et al. 2001. Biochem J. 354: 511-9. PMID: 11237855
4. From Bcg/Lsh/Ity to Nramp1: three decades of search and research.  |  Buschman, E. and Skamene, E. 2001. Drug Metab Dispos. 29: 471-3. PMID: 11259334
5. Allelic association between the NRAMP1 gene and susceptibility to tuberculosis in Guinea-Conakry.  |  Cervino, AC., et al. 2000. Ann Hum Genet. 64: 507-12. PMID: 11281214
6. Cloning and characterization of a second human NRAMP gene on chromosome 12q13.  |  Vidal, S., et al. 1995. Mamm Genome. 6: 224-30. PMID: 7613023
7. Human natural resistance-associated macrophage protein: cDNA cloning, chromosomal mapping, genomic organization, and tissue-specific expression.  |  Cellier, M., et al. 1994. J Exp Med. 180: 1741-52. PMID: 7964458
8. Susceptibility to leprosy is linked to the human NRAMP1 gene.  |  Abel, L., et al. 1998. J Infect Dis. 177: 133-45. PMID: 9419180
9. Variations in the NRAMP1 gene and susceptibility to tuberculosis in West Africans.  |  Bellamy, R., et al. 1998. N Engl J Med. 338: 640-4. PMID: 9486992
10. The human Nramp2 gene: characterization of the gene structure, alternative splicing, promoter region and polymorphisms.  |  Lee, PL., et al. 1998. Blood Cells Mol Dis. 24: 199-215. PMID: 9642100
11. The G185R mutation disrupts function of the iron transporter Nramp2.  |  Su, MA., et al. 1998. Blood. 92: 2157-63. PMID: 9731075
12. Human natural resistance-associated macrophage protein 2: gene cloning and protein identification.  |  Kishi, F. and Tabuchi, M. 1998. Biochem Biophys Res Commun. 251: 775-83. PMID: 9790986

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NRAMP 2 Antibody (G-5) has 19 citations in a variety of scientific publications.

Primary antibodies like Anti-NRAMP 2 Antibody (G-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NRAMP 2 Antibody (G-5) Specifications

Alternate Product Name: NRAMP 2

Product Clone Name: G-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 461-568

Epitope Target: NRAMP 2

Epitope Species: human

Product Reactivity/Detection: NRAMP 2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 19 citations in various scientific publications

Available Conjugates/Forms for NRAMP 2 Antibody (G-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin