NPWBP Antibody (F-1): sc-393556
- NPWBP Antibody (F-1) is a mouse monoclonal IgG2a κ NPWBP antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 294-319 within an internal region of NPWBP of human origin
- NPWBP Antibody (F-1) is recommended for detection of NPWBP of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-NPWBP Antibody (F-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for NPWBP Antibody (F-1) for WB applications. These reagents are now offered in bundles with NPWBP Antibody (F-1) (see ordering information below).
QUICK LINKS
SEE ALSO...
NPWBP Antibody (F-1) is a mouse monoclonal IgG2a kappa light chain antibody that detects NPWBP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-NPWBP antibody (F-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NPWBP protein, also known as Npw38-binding protein, WW domain-binding protein 11, and SH3 domain-binding protein SNP70, consists of 641 amino acids and features two proline-rich regions that interact with the WW domain of PQBP-1, a transcription repressor linked to polyglutamine tract-containing transcription regulators. NPWBP protein is highly expressed in the kidney, pancreas, brain, placenta, heart, and skeletal muscle, predominantly residing in the nucleus with a granular heterogeneous distribution. During mitosis, NPWBP protein translocates to the cytoplasm. Nuclear localization of NPWBP protein is significant as NPWBP co-localizes with essential mRNA splicing factors, SC35 and U2 snRNP B, indicating a crucial role in pre-mRNA processing, which is vital for gene expression regulation and functional protein production.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
NPWBP Antibody (F-1) References:
- Npw38, a novel nuclear protein possessing a WW domain capable of activating basal transcription. | Komuro, A., et al. 1999. Nucleic Acids Res. 27: 1957-65. PMID: 10198427
- Association of two nuclear proteins, Npw38 and NpwBP, via the interaction between the WW domain and a novel proline-rich motif containing glycine and arginine. | Komuro, A., et al. 1999. J Biol Chem. 274: 36513-9. PMID: 10593949
- Functions of WW domains in the nucleus. | Sudol, M., et al. 2001. FEBS Lett. 490: 190-5. PMID: 11223034
- A nuclear SH3 domain-binding protein that colocalizes with mRNA splicing factors and intermediate filament-containing perinuclear networks. | Craggs, G., et al. 2001. J Biol Chem. 276: 30552-60. PMID: 11375989
- WW and SH3 domains, two different scaffolds to recognize proline-rich ligands. | Macias, MJ., et al. 2002. FEBS Lett. 513: 30-7. PMID: 11911877
- Novel interaction partners of the CD2BP2-GYF domain. | Kofler, M., et al. 2005. J Biol Chem. 280: 33397-402. PMID: 16000308
- Structure and function of the two tandem WW domains of the pre-mRNA splicing factor FBP21 (formin-binding protein 21). | Huang, X., et al. 2009. J Biol Chem. 284: 25375-87. PMID: 19592703
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NPWBP Antibody (F-1) | sc-393556 | 200 µg/ml | $322.00 | |||
NPWBP Antibody (F-1): m-IgG Fc BP-HRP Bundle | sc-530495 | 200 µg Ab; 10 µg BP | $361.00 | |||
NPWBP Antibody (F-1): m-IgGκ BP-HRP Bundle | sc-523995 | 200 µg Ab, 40 µg BP | $361.00 | |||
NPWBP Antibody (F-1): m-IgG2a BP-HRP Bundle | sc-547509 | 200 µg Ab; 10 µg BP | $361.00 | |||
NPWBP Antibody (F-1) AC | sc-393556 AC | 500 µg/ml, 25% agarose | $424.00 | |||
NPWBP Antibody (F-1) HRP | sc-393556 HRP | 200 µg/ml | $322.00 | |||
NPWBP Antibody (F-1) FITC | sc-393556 FITC | 200 µg/ml | $336.00 | |||
NPWBP Antibody (F-1) PE | sc-393556 PE | 200 µg/ml | $349.00 | |||
NPWBP Antibody (F-1) Alexa Fluor® 488 | sc-393556 AF488 | 200 µg/ml | $364.00 | |||
NPWBP Antibody (F-1) Alexa Fluor® 546 | sc-393556 AF546 | 200 µg/ml | $364.00 | |||
NPWBP Antibody (F-1) Alexa Fluor® 594 | sc-393556 AF594 | 200 µg/ml | $364.00 | |||
NPWBP Antibody (F-1) Alexa Fluor® 647 | sc-393556 AF647 | 200 µg/ml | $364.00 | |||
NPWBP Antibody (F-1) Alexa Fluor® 680 | sc-393556 AF680 | 200 µg/ml | $364.00 | |||
NPWBP Antibody (F-1) Alexa Fluor® 790 | sc-393556 AF790 | 200 µg/ml | $364.00 | |||
NPWBP (F-1) Neutralizing Peptide | sc-393556 P | 100 µg/0.5 ml | $69.00 |