Npl4 Antibody (D-1): sc-365796

Npl4 Antibody (D-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects Npl4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Npl4 antibody (D-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Npl4, consisting of 608 amino acids, is primarily located in the endoplasmic reticulum and nuclear membrane, where Npl4 plays a crucial role in the cellular quality control system. By forming a complex with p97/Cdc48p and Ufd1p, Npl4 is essential for recognizing and processing ubiquitinated inositol 1,4,5-trisphosphate receptors within the endoplasmic reticulum, facilitating their delivery to the proteasome for degradation. This function is vital for maintaining cellular homeostasis and regulating intracellular signaling pathways. Additionally, Npl4 features multiple post-translational modifications, including potential N-myristoylation and N-glycosylation sites, as well as phosphorylation sites, which may influence stability and interactions with other proteins. Notably, mutations in the NPL4 gene can lead to significant defects in nuclear envelope morphology and impairments in nuclear protein import and RNA export, underscoring Npl4′s importance in cellular function and integrity.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Npl4 Antibody (D-1) References:

1. A complex of mammalian ufd1 and npl4 links the AAA-ATPase, p97, to ubiquitin and nuclear transport pathways.  |  Meyer, HH., et al. 2000. EMBO J. 19: 2181-92. PMID: 10811609
2. Cloning and characterization of the gene encoding human NPL4, a protein interacting with the ubiquitin fusion-degradation protein (UFD1L).  |  Botta, A., et al. 2001. Gene. 275: 39-46. PMID: 11574150
3. The AAA ATPase p97/VCP interacts with its alternative co-factors, Ufd1-Npl4 and p47, through a common bipartite binding mechanism.  |  Bruderer, RM., et al. 2004. J Biol Chem. 279: 49609-16. PMID: 15371428
4. Ufd1 exhibits the AAA-ATPase fold with two distinct ubiquitin interaction sites.  |  Park, S., et al. 2005. Structure. 13: 995-1005. PMID: 16004872
5. Involvement of the p97-Ufd1-Npl4 complex in the regulated endoplasmic reticulum-associated degradation of inositol 1,4,5-trisphosphate receptors.  |  Alzayady, KJ., et al. 2005. J Biol Chem. 280: 34530-7. PMID: 16103111
6. Nuclear transport defects and nuclear envelope alterations are associated with mutation of the Saccharomyces cerevisiae NPL4 gene.  |  DeHoratius, C. and Silver, PA. 1996. Mol Biol Cell. 7: 1835-55. PMID: 8930904
7. Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking.  |  Fabre, E. and Hurt, E. 1997. Annu Rev Genet. 31: 277-313. PMID: 9442897

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Npl4 Antibody (D-1) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-Npl4 Antibody (D-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Npl4 Antibody (D-1) Specifications

Alternate Product Name: Npl4

Product Clone Name: D-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-300

Epitope Target: Npl4

Epitope Species: human

Product Reactivity/Detection: Npl4 isoforms 1 and 2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for Npl4 Antibody (D-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin