NOXO1 Antibody (F-5): sc-390927

NOXO1 Antibody (F-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects NOXO1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NOXO1 (F-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NOXO1, also known as NADPH oxidase organizer 1, plays a crucial role in the assembly and activation of NADPH oxidase complexes, which are essential for generating reactive oxygen species (ROS) involved in various cellular signaling pathways and immune responses. Located primarily in the cytoplasm, NOXO1 targets NADPH oxidase to specific subcellular compartments, ensuring ROS production occurs in the right context and at the right time. Such spatial regulation is particularly important as dysregulation of ROS can lead to oxidative stress and contribute to various diseases, including neurodegenerative disorders and cancer. NOXO1 is also involved in the synthesis of otoliths, crystalline structures in the inner ear that play a key role in the perception of gravity and balance. NOXO1 consists of 376 amino acids and has four isoforms produced through alternative splicing, highlighting functional diversity and importance in cellular processes.

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NOXO1 Antibody (F-5) References:

1. Two novel proteins activate superoxide generation by the NADPH oxidase NOX1.  |  Bánfi, B., et al. 2003. J Biol Chem. 278: 3510-3. PMID: 12473664
2. Proteins homologous to p47phox and p67phox support superoxide production by NAD(P)H oxidase 1 in colon epithelial cells.  |  Geiszt, M., et al. 2003. J Biol Chem. 278: 20006-12. PMID: 12657628
3. Novel human homologues of p47phox and p67phox participate in activation of superoxide-producing NADPH oxidases.  |  Takeya, R., et al. 2003. J Biol Chem. 278: 25234-46. PMID: 12716910
4. NOXO1, regulation of lipid binding, localization, and activation of Nox1 by the Phox homology (PX) domain.  |  Cheng, G. and Lambeth, JD. 2004. J Biol Chem. 279: 4737-42. PMID: 14617635
5. NOX3, a superoxide-generating NADPH oxidase of the inner ear.  |  Bánfi, B., et al. 2004. J Biol Chem. 279: 46065-72. PMID: 15326186
6. The NADPH oxidase Nox3 constitutively produces superoxide in a p22phox-dependent manner: its regulation by oxidase organizers and activators.  |  Ueno, N., et al. 2005. J Biol Chem. 280: 23328-39. PMID: 15824103
7. Alternative mRNA splice forms of NOXO1: differential tissue expression and regulation of Nox1 and Nox3.  |  Cheng, G. and Lambeth, JD. 2005. Gene. 356: 118-26. PMID: 15949904
8. Molecular interaction of NADPH oxidase 1 with betaPix and Nox Organizer 1.  |  Park, HS., et al. 2006. Biochem Biophys Res Commun. 339: 985-90. PMID: 16329988
9. Interaction between the SH3 domains and C-terminal proline-rich region in NADPH oxidase organizer 1 (Noxo1).  |  Yamamoto, A., et al. 2007. Biochem Biophys Res Commun. 352: 560-5. PMID: 17126813
10. NoxO1 Knockout Promotes Longevity in Mice.  |  Schader, T., et al. 2020. Antioxidants (Basel). 9: PMID: 32164269
11. CYLD destabilizes NoxO1 protein by promoting ubiquitination and regulates prostate cancer progression.  |  Haq, S., et al. 2022. Cancer Lett. 525: 146-157. PMID: 34742871
12. NoxO1 regulates EGFR signaling by its interaction with Erbin.  |  Hebchen, DM., et al. 2024. Redox Biol. 77: 103396. PMID: 39426288

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NOXO1 Antibody (F-5) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-NOXO1 Antibody (F-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NOXO1 Antibody (F-5) Specifications

Alternate Product Name: NOXO1

Product Clone Name: F-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 50-339

Epitope Target: NOXO1

Epitope Species: mouse

Product Reactivity/Detection: NOXO1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for NOXO1 Antibody (F-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin