Nox4 Antibody (C-3): sc-518092

Nox4 Antibody (C-3) is a mouse monoclonal IgG2b kappa light chain antibody specifically engineered to detect human Nox4 protein, also known as Renox, a vital component of the NADPH oxidase family. Nox4 plays a significant role in cellular redox signaling by generating reactive oxygen species, particularly superoxide anions, which are essential for regulating processes such as cell differentiation, proliferation, and responses to hypoxic conditions. Post-translational modifications, such as phosphorylation, regulate Nox4′s activity and facilitate interactions with proteins like p47-phox and p67-phox, which are necessary for assembling the active NADPH oxidase complex. Additionally, Nox4 interacts with key signaling proteins involved in pathways like TGF-β, highlighting its role in processes such as fibrosis and vascular remodeling. anti-Nox4 antibody (C-3) is recommended for use in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA), demonstrating reactivity with human Nox4 in these applications. Nox4 is abundantly expressed in the proximal convoluted tubule epithelial cells of the renal cortex, where Nox4 contributes to erythropoietin production, and is also found in fetal tissues, the placenta, glioblastoma cells, and vascular cells. In vascular cells, Nox4 expression is upregulated in response to Angiotensin II, underscoring Nox4′s importance in the vascular oxidative stress response and potential implications in cardiovascular diseases. Nox4 (C-3) antibody is recommended for researchers investigating the diverse functions and regulatory mechanisms of Nox4 in various biological contexts.

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Nox4 Antibody (C-3) References:

1. O2 sensing is preserved in mice lacking the gp91 phox subunit of NADPH oxidase.  |  Archer, SL., et al. 1999. Proc Natl Acad Sci U S A. 96: 7944-9. PMID: 10393927
2. The p67(phox) activation domain regulates electron flow from NADPH to flavin in flavocytochrome b(558).  |  Nisimoto, Y., et al. 1999. J Biol Chem. 274: 22999-3005. PMID: 10438466
3. Identification of renox, an NAD(P)H oxidase in kidney.  |  Geiszt, M., et al. 2000. Proc Natl Acad Sci U S A. 97: 8010-4. PMID: 10869423
4. A novel superoxide-producing NAD(P)H oxidase in kidney.  |  Shiose, A., et al. 2001. J Biol Chem. 276: 1417-23. PMID: 11032835
5. Homologs of gp91phox: cloning and tissue expression of Nox3, Nox4, and Nox5.  |  Cheng, G., et al. 2001. Gene. 269: 131-40. PMID: 11376945
6. Upregulation of the vascular NAD(P)H-oxidase isoforms Nox1 and Nox4 by the renin-angiotensin system in vitro and in vivo.  |  Wingler, K., et al. 2001. Free Radic Biol Med. 31: 1456-64. PMID: 11728818
7. p22phox is a critical component of the superoxide-generating NADH/NADPH oxidase system and regulates angiotensin II-induced hypertrophy in vascular smooth muscle cells.  |  Ushio-Fukai, M., et al. 1996. J Biol Chem. 271: 23317-21. PMID: 8798532

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Nox4 Antibody (C-3) has 11 citations in a variety of scientific publications.

Primary antibodies like Anti-Nox4 Antibody (C-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Nox4 Antibody (C-3) Specifications

Alternate Product Name: Nox4

Product Clone Name: C-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 382-409

Epitope Target: Nox4

Epitope Species: human

Product Reactivity/Detection: Nox4

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 11 citations in various scientific publications

Available Conjugates/Forms for Nox4 Antibody (C-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin