NOD9 Antibody (F-2): sc-374514

NOD9 Antibody (F-2) is a mouse monoclonal IgG2b kappa light chain antibody that detects NOD9 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). NOD9 (F-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NOD9, also known as NLRX1, NOD26, or NOD5, is a 975 amino acid outer mitochondrial membrane protein characterized by the presence of one NACHT domain and four leucine-rich repeats (LRRs). These LRRs, which consist of 20-30 amino acid motifs, form a hydrophobic α/β horseshoe fold that is crucial for protein-protein interactions, allowing NOD9 to effectively engage with various signaling molecules. NOD9 is predominantly expressed in heart, muscle, and mammary gland tissues, where NOD9 plays a vital role in antiviral signaling by inhibiting virus-induced helicases, thus serving as a negative regulator of antiviral responses. Notably, NOD9 exists in two isoforms due to alternative splicing, which may contribute to functional diversity in immune responses.

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NOD9 Antibody (F-2) References:

1. NODs: intracellular proteins involved in inflammation and apoptosis.  |  Inohara, N. and Nuñez, G. 2003. Nat Rev Immunol. 3: 371-82. PMID: 12766759
2. NOD-LRR proteins: role in host-microbial interactions and inflammatory disease.  |  Inohara,., et al. 2005. Annu Rev Biochem. 74: 355-83. PMID: 15952891
3. NLRX1 is a regulator of mitochondrial antiviral immunity.  |  Moore, CB., et al. 2008. Nature. 451: 573-7. PMID: 18200010
4. NLRX1 is a mitochondrial NOD-like receptor that amplifies NF-kappaB and JNK pathways by inducing reactive oxygen species production.  |  Tattoli, I., et al. 2008. EMBO Rep. 9: 293-300. PMID: 18219313
5. NLRX1: friend or foe?  |  Meylan, E. and Tschopp, J. 2008. EMBO Rep. 9: 243-5. PMID: 18311173
6. Innate immunity: squelching anti-viral signalling with NLRX1.  |  O'Neill, LA. 2008. Curr Biol. 18: R302-4. PMID: 18397740
7. Negative regulation of cytoplasmic RNA-mediated antiviral signaling.  |  Komuro, A., et al. 2008. Cytokine. 43: 350-8. PMID: 18703349
8. NLRX1 attenuates endoplasmic reticulum stress via STING in cardiac hypertrophy.  |  Mi, K., et al. 2024. Biochim Biophys Acta Mol Cell Res. 1871: 119852. PMID: 39357547
9. Insights into the structure of NLR family member X1: Paving the way for innovative drug discovery.  |  Jewell, S., et al. 2024. Comput Struct Biotechnol J. 23: 3506-3513. PMID: 39435340
10. An NLR family member X1 mutation (p.Arg707Cys) suppresses hepatitis B virus infection in hepatocytes and favors the interaction of retinoic acid-inducible gene 1 with mitochondrial antiviral signaling protein.  |  Jiao, Q., et al. 2024. Arch Virol. 169: 238. PMID: 39499386
11. NLRX1 and STING alleviate renal ischemia-reperfusion injury by regulating LC3 lipidation during mitophagy.  |  Liao, Y., et al. 2024. Exp Cell Res. 443: 114323. PMID: 39505095
12. NOD-like receptor X1 promotes autophagy and inactivates NLR family pyrin domain containing 3 inflammasome signaling by binding autophagy-related gene 5 to alleviate cerebral ischemia/reperfusion-induced neuronal injury.  |  Peng, Y., et al. 2024. J Neuropathol Exp Neurol.. PMID: 39707156

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NOD9 Antibody (F-2) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-NOD9 Antibody (F-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NOD9 Antibody (F-2) Specifications

Alternate Product Name: NLRX1

Product Clone Name: F-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 89-230

Epitope Target: NOD9

Epitope Species: human

Product Reactivity/Detection: NOD9

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for NOD9 Antibody (F-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin