NOBOX Antibody (D-3): sc-390016

NOBOX Antibody (D-3) is a mouse monoclonal IgG1 antibody that detects NOBOX in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NOBOX, or newborn ovary homeobox gene, plays a pivotal role in early ovarian folliculogenesis, specifically in the formation and maintenance of primordial follicles. NOBOX (D-3) antibody is crucial for studying oocyte development regulation, as NOBOX influences the expression of key genes such as Pou5f1 and GDF-9, which are essential for proper oogenesis. Mutations in the NOBOX gene can lead to nonsyndromic ovarian failure by impairing NOBOX′s ability to bind to its DNA-binding element, resulting in disrupted gene regulation. While NOBOX expression is primarily oocyte-specific in the ovary, NOBOX is also found in the adult testis and pancreas, indicating potential involvement in other biological processes. Anti-NOBOX antibody (D-3) effectively detects this important protein, making NOBOX (D-3) monoclonal antibody a valuable tool for researchers studying ovarian function and related disorders.

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NOBOX Antibody (D-3) References:

1. Nobox is a homeobox-encoding gene preferentially expressed in primordial and growing oocytes.  |  Suzumori, N., et al. 2002. Mech Dev. 111: 137-41. PMID: 11804785
2. Characterization of NOBOX DNA binding specificity and its regulation of Gdf9 and Pou5f1 promoters.  |  Choi, Y. and Rajkovic, A. 2006. J Biol Chem. 281: 35747-56. PMID: 16997917
3. NOBOX homeobox mutation causes premature ovarian failure.  |  Qin, Y., et al. 2007. Am J Hum Genet. 81: 576-81. PMID: 17701902
4. New NOBOX mutations identified in a large cohort of women with primary ovarian insufficiency decrease KIT-L expression.  |  Bouilly, J., et al. 2015. J Clin Endocrinol Metab. 100: 994-1001. PMID: 25514101
5. NOBOX is a strong autosomal candidate gene in Tunisian patients with primary ovarian insufficiency.  |  Bouali, N., et al. 2016. Clin Genet. 89: 608-13. PMID: 26848058
6. A homozygous NOBOX truncating variant causes defective transcriptional activation and leads to primary ovarian insufficiency.  |  Li, L., et al. 2017. Hum Reprod. 32: 248-255. PMID: 27836978
7. Compound heterozygous null mutations of NOBOX in sisters with delayed puberty and primary amenorrhea.  |  Sassi, A., et al. 2021. Mol Genet Genomic Med. 9: e1776. PMID: 34480423
8. Hypo-Hydroxymethylation of Nobox is Associated with Ovarian Dysfunction in Rat Offspring Exposed to Prenatal Hypoxia.  |  Yao, C., et al. 2022. Reprod Sci. 29: 1424-1436. PMID: 35257353
9. Sumoylation regulates functional properties of the oocyte transcription factors SOHLH1 and NOBOX.  |  Patton, BK., et al. 2023. FASEB J. 37: e22747. PMID: 36607631
10. NOBOX gene variants in premature ovarian insufficiency: ethnicity-dependent insights.  |  Jordan, P., et al. 2024. J Assist Reprod Genet. 41: 135-146. PMID: 37921973

The preferred NOBOX antibody is NOBOX Antibody (A-5): sc-514178.