NOBOX Antibody (A-5): sc-514178

NOBOX Antibody (A-5) is a mouse monoclonal IgG2b antibody that detects NOBOX protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NOBOX (A-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NOBOX protein plays a crucial role in early ovarian folliculogenesis, which is essential for the development of primordial follicles from germ cell clusters. This process is vital for female fertility, as disruptions can lead to conditions such as premature ovarian failure (POF), affecting approximately 1% of women and resulting in symptoms like amenorrhea and elevated serum gonadotropin levels. Mutations in NOBOX gene can impair binding to NOBOX DNA-binding element, thereby inhibiting the regulation of key factors like Pou5f1 and GDF-9, which are critical for oocyte development. While NOBOX expression is primarily oocyte-specific in the ovary, expression is also found in the adult testis and pancreas, indicating potential involvement in other reproductive and developmental processes.

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NOBOX Antibody (A-5) References:

1. Nobox is a homeobox-encoding gene preferentially expressed in primordial and growing oocytes.  |  Suzumori, N., et al. 2002. Mech Dev. 111: 137-41. PMID: 11804785
2. NOBOX deficiency disrupts early folliculogenesis and oocyte-specific gene expression.  |  Rajkovic, A., et al. 2004. Science. 305: 1157-9. PMID: 15326356
3. Mutational analysis of the homeobox region of the human NOBOX gene in Japanese women who exhibit premature ovarian failure.  |  Zhao, XX., et al. 2005. Fertil Steril. 83: 1843-4. PMID: 15950662
4. Genetics of early mammalian folliculogenesis.  |  Choi, Y. and Rajkovic, A. 2006. Cell Mol Life Sci. 63: 579-90. PMID: 16416028
5. cDNA cloning and expression of the human NOBOX gene in oocytes and ovarian follicles.  |  Huntriss, J., et al. 2006. Mol Hum Reprod. 12: 283-9. PMID: 16597639
6. Characterization of NOBOX DNA binding specificity and its regulation of Gdf9 and Pou5f1 promoters.  |  Choi, Y. and Rajkovic, A. 2006. J Biol Chem. 281: 35747-56. PMID: 16997917
7. Candidate genes for premature ovarian failure.  |  Suzumori, N., et al. 2007. Curr Med Chem. 14: 353-7. PMID: 17305537
8. Microarray analyses of newborn mouse ovaries lacking Nobox.  |  Choi, Y., et al. 2007. Biol Reprod. 77: 312-9. PMID: 17494914
9. NOBOX homeobox mutation causes premature ovarian failure.  |  Qin, Y., et al. 2007. Am J Hum Genet. 81: 576-81. PMID: 17701902
10. Novel NOBOX loss-of-function mutations account for 6.2% of cases in a large primary ovarian insufficiency cohort.  |  Bouilly, J., et al. 2011. Hum Mutat. 32: 1108-13. PMID: 21837770
11. The NOBOX protein becomes undetectable in developmentally competent antral and ovulated oocytes.  |  Belli, M., et al. 2013. Int J Dev Biol. 57: 35-9. PMID: 23585350
12. NOBOX is a key FOXL2 partner involved in ovarian folliculogenesis.  |  Bouilly, J., et al. 2014. J Mol Cell Biol. 6: 175-7. PMID: 24620032

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NOBOX Antibody (A-5) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-NOBOX Antibody (A-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NOBOX Antibody (A-5) Specifications

Alternate Product Name: NOBOX

Product Clone Name: A-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b lambda light chain

Amino Acids: 61-79

Epitope Target: NOBOX

Epitope Species: mouse

Product Reactivity/Detection: NOBOX

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b lambda light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for NOBOX Antibody (A-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin