NNT Antibody (G-8) recommended for detection of NNT of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

NNT Antibody (G-8): sc-390215

NNT Antibody (G-8) is rated 5.0 out of 5 by 2.
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5
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Datasheets

    • NNT Antibody (G-8) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 787-1086 mapping at the C-terminus of NNT of human origin
    • recommended for detection of NNT of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and canine, bovine and porcine
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.

I am using NNT (G-8): sc-390215 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Cweed
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Superb immunofluorescence mitochondrial staining Superb immunofluorescence mitochondrial staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-03-07
Rated 5 out of 5 by from Good Western Blot data of NNT expression Good Western Blot data of NNT expression in Hep G2 and SW-13 whole cell lysates. -SCBT QC
Date published: 2013-11-21
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