NNT Antibody (B-3): sc-390236

NNT Antibody (B-3) is a mouse monoclonal IgG2b kappa light chain antibody that detects NNT protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-NNT antibody (B-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Nicotinamide nucleotide transhydrogenase (NNT) is a crucial multi-pass mitochondrial inner membrane protein composed of 1086 amino acids, functioning as a homodimer. NNT plays a vital role in cellular respiration by catalyzing the transfer of a hydride ion from NADH to NADP+, effectively acting as a proton pump within the mitochondrial inner membrane. This process is essential for generating high concentrations of NADPH, which are critical for free radical detoxification and various biosynthetic pathways. The unique structure of NNT, with its N-terminal section belonging to the AlaDH/PNT family and its C-terminal section to the PNT beta subunit family, underscores its importance in maintaining cellular energy balance and redox homeostasis. By utilizing the energy from the proton gradient established by the electron transport chain, NNT supports metabolic processes and protects cells from oxidative stress, making NNT a significant target for research in mitochondrial function and related diseases.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

NNT Antibody (B-3) References:

1. The high-resolution structure of the NADP(H)-binding component (dIII) of proton-translocating transhydrogenase from human heart mitochondria.  |  White, SA., et al. 2000. Structure. 8: 1-12. PMID: 10673423
2. Expression of proton-pumping nicotinamide nucleotide transhydrogenase in mouse, human brain and C elegans.  |  Arkblad, EL., et al. 2002. Comp Biochem Physiol B Biochem Mol Biol. 133: 13-21. PMID: 12223207
3. Proton translocation by transhydrogenase.  |  Jackson, JB. 2003. FEBS Lett. 545: 18-24. PMID: 12788487
4. The cDNA sequence of proton-pumping nicotinamide nucleotide transhydrogenase from man and mouse.  |  Arkblad, EL., et al. 1996. Biochim Biophys Acta. 1273: 203-5. PMID: 8616157
5. Oxidative modification of nicotinamide nucleotide transhydrogenase in submitochondrial particles: effect of endogenous ubiquinol.  |  Forsmark-Andrée, P., et al. 1996. Arch Biochem Biophys. 336: 113-20. PMID: 8951041
6. Cloning and deduced amino acid sequence of human nicotinamide nucleotide transhydrogenase.  |  Zieger, B. and Ware, J. 1997. DNA Seq. 7: 369-73. PMID: 9524818

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NNT Antibody (B-3) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-NNT Antibody (B-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NNT Antibody (B-3) Specifications

Alternate Product Name: NNT

Product Clone Name: B-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 787-1086

Epitope Target: NNT

Epitope Species: human

Product Reactivity/Detection: NNT

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: bovine, porcine and canine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for NNT Antibody (B-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin