NMNAT-2 Antibody (B-10): sc-515206

NMNAT-2 Antibody (B-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects NMNAT-2 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NMNAT-2 Antibody (B-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NMNAT-2, also known as nicotinamide mononucleotide adenylyltransferase 2, plays a crucial role in cellular metabolism by catalyzing the synthesis of NAD+ from nicotinamide mononucleotide and ATP, a process vital for energy production and cellular function. This 307 amino acid protein is predominantly expressed in the brain, particularly in regions such as the cerebrum, cerebellum, and various lobes, highlighting its importance in neurological health. Additionally, NMNAT-2 undergoes post-translational modifications, including phosphorylation, which can influence interactions with nuclear proteins and potentially modulate enzymatic activity. Multiple phosphorylation sites suggest NMNAT-2 regulation by various kinases, further emphasizing significance in cellular signaling pathways.

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NMNAT-2 Antibody (B-10) References:

1. Age-dependent synapse withdrawal at axotomised neuromuscular junctions in Wld(s) mutant and Ube4b/Nmnat transgenic mice.  |  Gillingwater, TH., et al. 2002. J Physiol. 543: 739-55. PMID: 12231635
2. Identification of a novel human nicotinamide mononucleotide adenylyltransferase.  |  Raffaelli, N., et al. 2002. Biochem Biophys Res Commun. 297: 835-40. PMID: 12359228
3. Subcellular compartmentation and differential catalytic properties of the three human nicotinamide mononucleotide adenylyltransferase isoforms.  |  Berger, F., et al. 2005. J Biol Chem. 280: 36334-41. PMID: 16118205
4. Regulation of poly(ADP-ribose) polymerase 1 activity by the phosphorylation state of the nuclear NAD biosynthetic enzyme NMN adenylyl transferase 1.  |  Berger, F., et al. 2007. Proc Natl Acad Sci U S A. 104: 3765-70. PMID: 17360427
5. Initial-rate kinetics of human NMN-adenylyltransferases: substrate and metal ion specificity, inhibition by products and multisubstrate analogues, and isozyme contributions to NAD+ biosynthesis.  |  Sorci, L., et al. 2007. Biochemistry. 46: 4912-22. PMID: 17402747
6. NMNAT expression and its relation to NAD metabolism.  |  Jayaram, HN., et al. 2011. Curr Med Chem. 18: 1962-72. PMID: 21517776
7. The NAD+ synthesizing enzyme nicotinamide mononucleotide adenylyltransferase 2 (NMNAT-2) is a p53 downstream target.  |  Pan, LZ., et al. 2014. Cell Cycle. 13: 1041-8. PMID: 24552824
8. 2'-Deoxyadenosine 5'-diphosphoribose is an endogenous TRPM2 superagonist.  |  Fliegert, R., et al. 2017. Nat Chem Biol. 13: 1036-1044. PMID: 28671679
9. Metabolic regulation of transcription through compartmentalized NAD+ biosynthesis.  |  Ryu, KW., et al. 2018. Science. 360: PMID: 29748257
10. EGCG inhibits pressure overload-induced cardiac hypertrophy via the PSMB5/Nmnat2/SIRT6-dependent signalling pathways.  |  Cai, Y., et al. 2021. Acta Physiol (Oxf). 231: e13602. PMID: 33315278
11. Ribosome ADP-ribosylation inhibits translation and maintains proteostasis in cancers.  |  Challa, S., et al. 2021. Cell. 184: 4531-4546.e26. PMID: 34314702
12. Ribosome ADP-ribosylation: A mechanism for maintaining protein homeostasis in cancers.  |  Ni, J., et al. 2022. Cell Biol Int. 46: 333-335. PMID: 34897867

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NMNAT-2 Antibody (B-10) has 16 citations in a variety of scientific publications.

Primary antibodies like Anti-NMNAT-2 Antibody (B-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NMNAT-2 Antibody (B-10) Specifications

Alternate Product Name: NMNAT-2

Product Clone Name: B-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 208-307

Epitope Target: NMNAT-2

Epitope Species: human

Product Reactivity/Detection: NMNAT-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 16 citations in various scientific publications

Available Conjugates/Forms for NMNAT-2 Antibody (B-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin