nm23-H2 Antibody (X-42): sc-100400

nm23-H2 Antibody (X-42) is a mouse monoclonal IgG1 kappa light chain antibody that detects nm23-H2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). anti-nm23-H2 antibody (X-42) is available as the non-conjugated form. The nm23 gene is recognized as a potential suppressor of metastasis, having been initially identified through differential hybridization between two murine melanoma sub-lines, one exhibiting high metastatic potential and the other low. Notably, highly metastatic sub-lines show significantly reduced levels of nm23 compared to their less metastatic counterparts. nm23-H2 plays a crucial role in cellular processes, including involvement in nucleotide metabolism, as nm23-H2 is closely related to nucleotide diphosphate kinases (NDP). In humans, NDP kinases A and B correspond to two isotypes of human nm23 homologs, nm23-H1 and H2, respectively. Furthermore, nm23-H2 is identical in sequence to PuF, a transcription factor that binds to specific elements within the human C-Myc promoter, which is essential for regulating gene expression linked to cell proliferation and growth. This interaction underscores nm23-H2′s importance in cellular signaling pathways and potential implications in cancer biology, making nm23-H2 monoclonal antibody (X-42) a valuable tool for researchers studying metastasis and transcriptional regulation.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

nm23-H2 Antibody (X-42) References:

1. Secretion of a nucleoside diphosphate kinase (Nm23-H2) by cells from human breast, colon, pancreas and lung tumors.  |  Anzinger, J., et al. 2001. Proc West Pharmacol Soc. 44: 61-3. PMID: 11793996
2. Molecular cloning and functional expression of the second mouse nm23/NDP kinase gene, nm23-M2.  |  Urano, T., et al. 1992. FEBS Lett. 309: 358-62. PMID: 1325378
3. Identification of NM23-H2 as a tumour-associated antigen in chronic myeloid leukaemia.  |  Tschiedel, S., et al. 2008. Leukemia. 22: 1542-50. PMID: 18496563
4. Identification of a second human nm23 gene, nm23-H2.  |  Stahl, JA., et al. 1991. Cancer Res. 51: 445-9. PMID: 1988104
5. Functional cloning of a nucleoside diphosphate kinase from Dictyostelium discoideum.  |  Lacombe, ML., et al. 1990. J Biol Chem. 265: 10012-8. PMID: 2161830
6. Isolation and characterization of a cDNA clone encoding rat nucleoside diphosphate kinase.  |  Kimura, N., et al. 1990. J Biol Chem. 265: 15744-9. PMID: 2168422
7. Recruited metastasis suppressor NM23-H2 attenuates expression and activity of peroxisome proliferator-activated receptor δ (PPARδ) in human cholangiocarcinoma.  |  He, F., et al. 2015. Dig Liver Dis. 47: 62-7. PMID: 25277864
8. Regulatory functions of Nm23-H2 in tumorigenesis: insights from biochemical to clinical perspectives.  |  Li, Y., et al. 2015. Naunyn Schmiedebergs Arch Pharmacol. 388: 243-56. PMID: 25413836
9. Mix and match of the tumor metastasis suppressor Nm23 protein isoforms in vitro and in vivo.  |  Potel, CM., et al. 2018. FEBS J. 285: 2856-2868. PMID: 29863788
10. Evidence for a novel gene associated with low tumor metastatic potential.  |  Steeg, PS., et al. 1988. J Natl Cancer Inst. 80: 200-4. PMID: 3346912
11. Expression of nm23/NDP kinase proteins on the cell surface.  |  Urano, T., et al. 1993. Oncogene. 8: 1371-6. PMID: 8386830
12. Human c-myc transcription factor PuF identified as nm23-H2 nucleoside diphosphate kinase, a candidate suppressor of tumor metastasis.  |  Postel, EH., et al. 1993. Science. 261: 478-80. PMID: 8392752

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-nm23-H2 Antibody (X-42) has 11 citations in a variety of scientific publications.

Primary antibodies like Anti-nm23-H2 Antibody (X-42) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

nm23-H2 Antibody (X-42) Specifications

Alternate Product Name: nm23-H2

Product Clone Name: X-42

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-153

Epitope Target: nm23-H2

Epitope Species: human

Product Reactivity/Detection: nm23-H2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 11 citations in various scientific publications

Available Conjugates/Forms for nm23-H2 Antibody (X-42): N/A

The preferred nm23-H1/2/3 antibody is nm23-H1/2/3 Antibody (E-11): sc-166937.