NKCC1 Antibody (F-4): sc-514858

NKCC1 Antibody (F-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects NKCC1 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). NKCC1 (F-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NKCC1 plays a crucial role in transcellular movement of chloride ions across epithelial tissues, essential for maintaining fluid balance and electrolyte homeostasis in the body. NKCC1 is predominantly expressed in muscle cells, neurons, and red blood cells, facilitating active chloride secretion in basolateral membrane of secretory epithelia. The gene encoding human NKCC1 is located on chromosome 5q23.3, and NKCC1 disruption in mice has been linked to deafness and balance impairments, highlighting NKCC1′s importance in physiological functions. NKCC1 is part of a larger family of Na-K-Cl cotransporters, including NKCC2, primarily expressed in kidney and involved in sodium chloride reabsorption, and NCCT, which is sensitive to thiazide diuretics and plays a significant role in renal sodium reabsorption. Understanding NKCC1 function and regulation is vital for developing therapeutic strategies for conditions related to electrolyte imbalances and fluid retention.

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NKCC1 Antibody (F-4) References:

1. NKCC1 and hypertension: role in the regulation of vascular smooth muscle contractions and myogenic tone.  |  Orlov, SN., et al. 2012. Ann Med. 44 Suppl 1: S111-8. PMID: 22713139
2. NKCC1 Chloride Importer Antagonists Attenuate Many Neurological and Psychiatric Disorders.  |  Ben-Ari, Y. 2017. Trends Neurosci. 40: 536-554. PMID: 28818303
3. Role of NKCC1 and KCC2 in Epilepsy: From Expression to Function.  |  Liu, R., et al. 2019. Front Neurol. 10: 1407. PMID: 32010056
4. NKCC1, an Elusive Molecular Target in Brain Development: Making Sense of the Existing Data.  |  Virtanen, MA., et al. 2020. Cells. 9: PMID: 33291778
5. Choroid plexus NKCC1 mediates cerebrospinal fluid clearance during mouse early postnatal development.  |  Xu, H., et al. 2021. Nat Commun. 12: 447. PMID: 33469018
6. High-Resolution Views and Transport Mechanisms of the NKCC1 and KCC Transporters.  |  Chew, TA., et al. 2021. J Mol Biol. 433: 167056. PMID: 34022207
7. Role of SPAK-NKCC1 signaling cascade in the choroid plexus blood-CSF barrier damage after stroke.  |  Wang, J., et al. 2022. J Neuroinflammation. 19: 91. PMID: 35413993
8. Expression patterns of NKCC1 in neurons and non-neuronal cells during cortico-hippocampal development.  |  Kurki, SN., et al. 2023. Cereb Cortex. 33: 5906-5923. PMID: 36573432
9. The role of KCC2 and NKCC1 in spinal cord injury: From physiology to pathology.  |  Talifu, Z., et al. 2022. Front Physiol. 13: 1045520. PMID: 36589461
10. Cation-Chloride Cotransporters KCC2 and NKCC1 as Therapeutic Targets in Neurological and Neuropsychiatric Disorders.  |  Lam, P., et al. 2023. Molecules. 28: PMID: 36771011

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NKCC1 Antibody (F-4) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-NKCC1 Antibody (F-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NKCC1 Antibody (F-4) Specifications

Alternate Product Name: SLC12A2

Product Clone Name: F-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 78-99

Epitope Target: NKCC1

Epitope Species: human

Product Reactivity/Detection: NKCC1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for NKCC1 Antibody (F-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred NKCC1 antibody is NKCC1 Antibody (A-6): sc-514774.