Nix Antibody (H-8): sc-166332

Nix Antibody (H-8) is a mouse monoclonal IgG2b lambda light chain antibody designed to target the Nix protein, also known as BNIP3L. Nix monoclonal antibody (H-8) is engineered to recognize an epitope mapping between amino acids 19-47 near the N-terminus of the human Nix protein, ensuring precise and reliable binding. Nix is a pro-apoptotic member of the Bcl-2 family, and its function is intricately regulated by post-translational modifications such as phosphorylation and ubiquitination. These modifications are critical for controlling Nix′s stability, subcellular localization, and interactions with other proteins involved in apoptotic signaling pathways. The structural integrity and regulation of Nix through these modifications enable Nix to effectively localize to the mitochondria, where Nix interacts with anti-apoptotic proteins like Bcl-xL to initiate programmed cell death, a vital process for maintaining cellular homeostasis. Anti-Nix antibody (H-8) exhibits broad species reactivity, effectively recognizing Nix proteins from mouse, rat, and human origins across various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Available in both non-conjugated and multiple conjugated forms such as horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, Nix (H-8) antibody offers versatile options to suit diverse experimental needs. By enabling the accurate detection and analysis of Nix and its post-translational modifications, anti-Nix antibody (H-8) supports advanced research into the molecular mechanisms of apoptosis and mitochondrial dynamics.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Nix Antibody (H-8) References:

1. Novel BNIP1 variants and their interaction with BCL2 family members.  |  Zhang, H., et al. 1999. FEBS Lett. 448: 23-7. PMID: 10217402
2. Tyrosine phosphorylation of the Bcl-2-associated protein BNIP-2 by fibroblast growth factor receptor-1 prevents its binding to Cdc42GAP and Cdc42.  |  Low, BC., et al. 1999. J Biol Chem. 274: 33123-30. PMID: 10551883
3. The role of hypoxia inducible factor 1alpha in cobalt chloride induced cell death in mouse embryonic fibroblasts.  |  Vengellur, A. and LaPres, JJ. 2004. Toxicol Sci. 82: 638-46. PMID: 15375294
4. Proapoptotic Nix activates the JNK pathway by interacting with POSH and mediates death in a Parkinson disease model.  |  Wilhelm, M., et al. 2007. J Biol Chem. 282: 1288-95. PMID: 17095503
5. Adenovirus E1B 19 kDa and Bcl-2 proteins interact with a common set of cellular proteins.  |  Boyd, JM., et al. 1994. Cell. 79: 341-51. PMID: 7954800
6. Functional complementation of the adenovirus E1B 19-kilodalton protein with Bcl-2 in the inhibition of apoptosis in infected cells.  |  Chiou, SK., et al. 1994. J Virol. 68: 6553-66. PMID: 8083992
7. Functional substitution identifies a cell survival promoting domain common to adenovirus E1B 19 kDa and Bcl-2 proteins.  |  Subramanian, T., et al. 1995. Oncogene. 11: 2403-9. PMID: 8570192
8. The E1B 19K/Bcl-2-binding protein Nip3 is a dimeric mitochondrial protein that activates apoptosis.  |  Chen, G., et al. 1997. J Exp Med. 186: 1975-83. PMID: 9396766
9. Nix and Nip3 form a subfamily of pro-apoptotic mitochondrial proteins.  |  Chen, G., et al. 1999. J Biol Chem. 274: 7-10. PMID: 9867803

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Nix Antibody (H-8) has 19 citations in a variety of scientific publications.

Primary antibodies like Anti-Nix Antibody (H-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Nix Antibody (H-8) Specifications

Alternate Product Name: Nix

Product Clone Name: H-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b lambda light chain

Amino Acids: 19-47

Epitope Target: Nix

Epitope Species: human

Product Reactivity/Detection: Nix

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b lambda light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 19 citations in various scientific publications

Available Conjugates/Forms for Nix Antibody (H-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred BNIP-3 antibody is BNIP-3 Antibody (ANa40): sc-56167.