BNIP-3 Antibody (ANa40): sc-56167
- BNIP-3 Antibody (ANa40) is a mouse monoclonal IgG2b κ BNIP-3 antibody, cited in 72 publications, provided at 200 µg/ml
- raised against amino acids 1-163 of BNIP-3 of human origin
- BNIP-3 Antibody (ANa40) is recommended for detection of BNIP-3 of mouse, rat and human origin by WB, IP and IF
- Anti-BNIP-3 Antibody (ANa40) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP is the preferred secondary detection reagent for BNIP-3 Antibody (ANa40) for WB applications. This reagent is now offered in a bundle with BNIP-3 Antibody (ANa40) (see ordering information below).
BNIP-3 Antibody (ANa40) is a mouse monoclonal IgG2b kappa light chain antibody engineered to detect BNIP-3, also known as NIP3, across mouse, rat, and human species using western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF) techniques. BNIP-3 monoclonal antibody (ANa40) targets the amino acids 1-163 region of the human BNIP-3 protein, ensuring precise binding and robust performance in various experimental settings. BNIP-3 is a critical member of the Bcl-2 family of proteins, which are essential regulators of apoptosis, the programmed cell death process vital for maintaining cellular homeostasis and eliminating damaged or unwanted cells. By interacting with Bcl-xL, BNIP-3 suppresses its anti-apoptotic activity, thereby promoting apoptosis, which is crucial in developmental processes, immune responses, and the prevention of cancerous growths. Understanding BNIP-3′s function is important for research into diseases where apoptosis is disrupted, such as cancer and neurodegenerative disorders. Anti-BNIP-3 antibody (ANa40) is available in both non-conjugated form and multiple conjugated forms, including horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, providing flexibility for diverse experimental applications. BNIP-3 monoclonal antibody (ANa40) cross-reacts with BNIP-3 from different species, making this antibody a versatile tool for comparative studies and translational research.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
BNIP-3 Antibody (ANa40) References:
- Novel BNIP1 variants and their interaction with BCL2 family members. | Zhang, H., et al. 1999. FEBS Lett. 448: 23-7. PMID: 10217402
- Tyrosine phosphorylation of the Bcl-2-associated protein BNIP-2 by fibroblast growth factor receptor-1 prevents its binding to Cdc42GAP and Cdc42. | Low, BC., et al. 1999. J Biol Chem. 274: 33123-30. PMID: 10551883
- Adenovirus E1B 19 kDa and Bcl-2 proteins interact with a common set of cellular proteins. | Boyd, JM., et al. 1994. Cell. 79: 341-51. PMID: 7954800
- Functional complementation of the adenovirus E1B 19-kilodalton protein with Bcl-2 in the inhibition of apoptosis in infected cells. | Chiou, SK., et al. 1994. J Virol. 68: 6553-66. PMID: 8083992
- Functional substitution identifies a cell survival promoting domain common to adenovirus E1B 19 kDa and Bcl-2 proteins. | Subramanian, T., et al. 1995. Oncogene. 11: 2403-9. PMID: 8570192
- The E1B 19K/Bcl-2-binding protein Nip3 is a dimeric mitochondrial protein that activates apoptosis. | Chen, G., et al. 1997. J Exp Med. 186: 1975-83. PMID: 9396766
- Nix and Nip3 form a subfamily of pro-apoptotic mitochondrial proteins. | Chen, G., et al. 1999. J Biol Chem. 274: 7-10. PMID: 9867803
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BNIP-3 Antibody (ANa40) | sc-56167 | 200 µg/ml | $316.00 | |||
BNIP-3 Antibody (ANa40): m-IgG2b BP-HRP Bundle | sc-548658 | 200 µg Ab; 10 µg BP | $354.00 | |||
BNIP-3 Antibody (ANa40) AC | sc-56167 AC | 500 µg/ml, 25% agarose | $416.00 | |||
BNIP-3 Antibody (ANa40) HRP | sc-56167 HRP | 200 µg/ml | $316.00 | |||
BNIP-3 Antibody (ANa40) FITC | sc-56167 FITC | 200 µg/ml | $330.00 | |||
BNIP-3 Antibody (ANa40) PE | sc-56167 PE | 200 µg/ml | $343.00 | |||
BNIP-3 Antibody (ANa40) Alexa Fluor® 488 | sc-56167 AF488 | 200 µg/ml | $357.00 | |||
BNIP-3 Antibody (ANa40) Alexa Fluor® 546 | sc-56167 AF546 | 200 µg/ml | $357.00 | |||
BNIP-3 Antibody (ANa40) Alexa Fluor® 594 | sc-56167 AF594 | 200 µg/ml | $357.00 | |||
BNIP-3 Antibody (ANa40) Alexa Fluor® 647 | sc-56167 AF647 | 200 µg/ml | $357.00 | |||
BNIP-3 Antibody (ANa40) Alexa Fluor® 680 | sc-56167 AF680 | 200 µg/ml | $357.00 | |||
BNIP-3 Antibody (ANa40) Alexa Fluor® 790 | sc-56167 AF790 | 200 µg/ml | $357.00 |