Nix Antibody (E-1): sc-166314

Nix Antibody (E-1) is a mouse monoclonal IgG1 antibody designed to target the Nix protein, also known as Bnip3L. Anti-Nix antibody (E-1) is engineered to recognize an epitope mapping between amino acids 19-47 near the N-terminus of the human Nix protein, ensuring precise and reliable binding. Nix is a pro-apoptotic member of the Bcl-2 family, characterized by distinct structural features, including a BH3 domain that is essential for interaction with other Bcl-2 proteins. The structural integrity of Nix facilitates localization to the mitochondria, a key cellular organelle involved in regulating apoptosis and maintaining mitochondrial integrity. Within the mitochondria, Nix plays a vital role in promoting apoptotic signaling by interacting with anti-apoptotic proteins such as Bcl-xL, thereby initiating the pathways necessary for programmed cell death and cellular homeostasis. Nix monoclonal antibody (E-1) exhibits broad species reactivity, effectively recognizing Nix proteins from mouse, rat, and human origins across various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). This versatility makes Nix (E-1) antibody an indispensable tool for researchers studying apoptosis, mitochondrial dynamics, and related signaling mechanisms in different model organisms. Anti-Nix antibody (E-1) supports advancements in understanding the molecular pathways of cell death and their implications in diseases such as cancer and neurodegenerative disorders.

The preferred BNIP-3 antibody is BNIP-3 Antibody (ANa40): sc-56167.