Nitrotyrosine Antibody (6D611): sc-71705

Nitrotyrosine Antibody (6D611) is a mouse monoclonal IgG2a antibody that detects nitrotyrosine in non-species reactive samples by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Nitrotyrosine is a critical biomarker for inflammation and nitric oxide (NO) production, formed in the presence of the active metabolite NO. Anti-nitrotyrosine antibody (6D611) recognizes a stable product of multiple pathways, including the formation of peroxynitrite, which can lead to cellular damage. The presence of nitrotyrosine in plasma is increasingly recognized as a valuable indicator of NO-dependent damage in vivo, making nitrotyrosine essential for understanding inflammatory processes. Elevated levels of nitrotyrosine have been associated with several inflammatory diseases, including septic shock, rheumatoid arthritis, celiac disease, atherosclerotic plaques, and chronic renal failure, highlighting nitrotyrosine′s importance in both diagnostic and therapeutic contexts. Nitrotyrosine monoclonal antibody (6D611) provides researchers with a powerful tool to investigate these conditions and further explore the underlying mechanisms of inflammation and oxidative stress.

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Nitrotyrosine Antibody (6D611) References:

1. Analytical methods for 3-nitrotyrosine as a marker of exposure to reactive nitrogen species: a review.  |  Herce-Pagliai, C., et al. 1998. Nitric Oxide. 2: 324-36. PMID: 10100488
2. Increased nitrotyrosine in exhaled breath condensate in cystic fibrosis.  |  Balint, B., et al. 2001. Eur Respir J. 17: 1201-7. PMID: 11491165
3. Nitrotyrosine and nitrate/nitrite levels in cardiac arrest survivors treated with endovascular hypothermia.  |  Krüger, A., et al. 2012. Physiol Res. 61: 425-30. PMID: 22670696
4. Nitrotyrosine level was associated with mortality in patients with acute kidney injury.  |  Qian, J., et al. 2013. PLoS One. 8: e79962. PMID: 24278225
5. Establishing 3-nitrotyrosine as a biomarker for the vasculopathy of Fabry disease.  |  Shu, L., et al. 2014. Kidney Int. 86: 58-66. PMID: 24402087
6. Proteomic analysis of 4-hydroxynonenal and nitrotyrosine modified proteins in RTT fibroblasts.  |  Pecorelli, A., et al. 2016. Int J Biochem Cell Biol. 81: 236-245. PMID: 27495376
7. Nitrotyrosine (NT), a Nitrosative Stress Biomarker, Plasma Concentrations in Gallstone Disease and Cancer Patients.  |  Saimanen, I., et al. 2019. Anticancer Res. 39: 809-814. PMID: 30711961
8. Effect of serum 3-nitrotyrosine on the occurrence and development of carotid atherosclerosis in patients with essential hypertension.  |  Zhao, J., et al. 2021. Minerva Med. 112: 670-671. PMID: 32338481
9. iNOS expressing macrophages co-localize with nitrotyrosine staining after myocardial infarction in humans.  |  Wilmes, V., et al. 2023. Front Cardiovasc Med. 10: 1104019. PMID: 37063955
10. Nitrotyrosine residues in placenta. Evidence of peroxynitrite formation and action.  |  Myatt, L., et al. 1996. Hypertension. 28: 488-93. PMID: 8794838

The preferred Nitrotyrosine antibody is Nitrotyrosine Antibody (39B6): sc-32757.