Nitrotyrosine Antibody (39B6): sc-32757

Nitrotyrosine Antibody (39B6) is a mouse monoclonal IgG2a kappa light chain antibody that detects Nitrotyrosine in non-species reactive applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Anti-Nitrotyrosine antibody (39B6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Nitrotyrosine serves as a critical biomarker for inflammation and nitric oxide (NO) production, forming when tyrosine residues are nitrosylated in the presence of reactive nitrogen species. Nitrotyrosine presence indicates oxidative stress and can be a stable product of various pathological processes, including peroxynitrite formation. Elevated Nitrotyrosine levels have been associated with several inflammatory conditions, such as septic shock, rheumatoid arthritis, celiac disease, atherosclerotic plaques, and chronic renal failure, making Nitrotyrosine an important target for research into these diseases. Understanding Nitrotyrosine′s role in these contexts can provide insights into NO-dependent damage mechanisms and overall inflammatory response, highlighting Nitrotyrosine′s relevance in both clinical and research settings.

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Nitrotyrosine Antibody (39B6) References:

1. iNOS and nitrotyrosine expression after spinal cord injury.  |  Xu, J., et al. 2001. J Neurotrauma. 18: 523-32. PMID: 11393255
2. Immunodetection of 3-nitrotyrosine in the liver of zymosan-treated rats with a new monoclonal antibody: comparison to analysis by HPLC.  |  Girault, I., et al. 2001. Free Radic Biol Med. 31: 1375-87. PMID: 11728809
3. Nitric oxide, nitrotyrosine, and nitric oxide modulators in asthma and chronic obstructive pulmonary disease.  |  Kharitonov, SA. and Barnes, PJ. 2003. Curr Allergy Asthma Rep. 3: 121-9. PMID: 12562551
4. Immunohistochemical artifact for nitrotyrosine in eosinophils or eosinophil containing tissue.  |  Ogino, K., et al. 2002. Free Radic Res. 36: 1163-70. PMID: 12592668
5. Prevention of peroxynitrite-induced renal injury through modulation of peroxynitrite production by the Chinese prescription Wen-Pi-Tang.  |  Rhyu, DY., et al. 2002. Free Radic Res. 36: 1261-9. PMID: 12607816
6. Plasma 3-nitrotyrosine and outcome in neonates with severe bronchopulmonary dysplasia after inhaled nitric oxide.  |  Lorch, SA., et al. 2003. Free Radic Biol Med. 34: 1146-52. PMID: 12706495
7. Circulating free nitrotyrosine and cognitive decline.  |  Coppola, L., et al. 2010. Acta Neurol Scand. 122: 175-81. PMID: 20003087
8. A Method for Analysis of Nitrotyrosine-Containing Proteins by Immunoblotting Coupled with Mass Spectrometry.  |  Kohutiar, M. and Eckhardt, A. 2021. Methods Mol Biol. 2276: 383-396. PMID: 34060056
9. Elevated free nitrotyrosine levels, but not protein-bound nitrotyrosine or hydroxyl radicals, throughout amyotrophic lateral sclerosis (ALS)-like disease implicate tyrosine nitration as an aberrant in vivo property of one familial ALS-linked superoxide dismutase 1 mutant.  |  Bruijn, LI., et al. 1997. Proc Natl Acad Sci U S A. 94: 7606-11. PMID: 9207139
10. Presence of inducible nitric oxide synthase, nitrotyrosine, CD68, and CD14 in the small intestine in celiac disease.  |  ter Steege, J., et al. 1997. Lab Invest. 77: 29-36. PMID: 9251676
11. Nitrotyrosine in plasma of celiac disease patients as detected by a new sandwich ELISA.  |  ter Steege, JC., et al. 1998. Free Radic Biol Med. 25: 953-63. PMID: 9840741
12. Immunohistochemical methods to detect nitrotyrosine.  |  Viera, L., et al. 1999. Methods Enzymol. 301: 373-81. PMID: 9919586

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Nitrotyrosine Antibody (39B6) has 156 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Nitrotyrosine Antibody (39B6) Specifications

Alternate Product Name: Nitrotyrosine

Product Clone Name: 39B6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: 3-Nitrotyrosine

Epitope Species:

Product Reactivity/Detection: nitrosylated tyrosine containing proteins

Species Reactivity/Detection: NSR

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 156 citations in various scientific publications

Available Conjugates/Forms for Nitrotyrosine Antibody (39B6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin