nicastrin Antibody (B-3): sc-376513

nicastrin Antibody (B-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects nicastrin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-nicastrin antibody (B-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. nicastrin plays a crucial role in the processing of the β-Amyloid precursor protein (βAPP) and is a key component of the presenilin complex, which is essential for the regulated intramembrane proteolysis of several proteins, including Notch. The interaction of nicastrin with presenilin proteins facilitates the cleavage of βAPP, leading to the production of β-Amyloid peptides, which are implicated in the pathogenesis of Alzheimer′s disease. Given that mutations in presenilin genes can enhance βAPP processing and contribute to neurotoxic peptide accumulation, nicastrin′s involvement in this pathway makes nicastrin a significant target for therapeutic strategies aimed at reducing β-Amyloid levels in Alzheimer′s patients. Originally purified from immunoprecipitated presenilin 1 complexes in HEK293 cells, nicastrin is characterized by a type I transmembrane glycoprotein structure, which includes hydrophilic amino and carboxy-terminal domains, a short hydrophobic transmembrane domain, and potential sites for N-myristoylation and phosphorylation, underscoring nicastrin′s functional versatility and importance in cellular signaling pathways.

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nicastrin Antibody (B-3) References:

1. A presenilin-1-dependent gamma-secretase-like protease mediates release of Notch intracellular domain.  |  De Strooper, B., et al. 1999. Nature. 398: 518-22. PMID: 10206645
2. Proteolytic release and nuclear translocation of Notch-1 are induced by presenilin-1 and impaired by pathogenic presenilin-1 mutations.  |  Song, W., et al. 1999. Proc Natl Acad Sci U S A. 96: 6959-63. PMID: 10359821
3. Presenilins: molecular switches between proteolysis and signal transduction.  |  Annaert, W. and De Strooper, B. 1999. Trends Neurosci. 22: 439-43. PMID: 10481190
4. Separation of presenilin function in amyloid beta-peptide generation and endoproteolysis of Notch.  |  Kulic, L., et al. 2000. Proc Natl Acad Sci U S A. 97: 5913-8. PMID: 10811883
5. Nicastrin modulates presenilin-mediated notch/glp-1 signal transduction and betaAPP processing.  |  Yu, G., et al. 2000. Nature. 407: 48-54. PMID: 10993067
6. Nicastrin is required for assembly of presenilin/gamma-secretase complexes to mediate Notch signaling and for processing and trafficking of beta-amyloid precursor protein in mammals.  |  Li, T., et al. 2003. J Neurosci. 23: 3272-7. PMID: 12716934
7. Structure of nicastrin unveils secrets of γ-secretase.  |  Bolduc, DM. and Wolfe, MS. 2014. Proc Natl Acad Sci U S A. 111: 14643-4. PMID: 25267656
8. Structure of the transmembrane domain of human nicastrin-a component of γ-secretase.  |  Li, Y., et al. 2016. Sci Rep. 6: 19522. PMID: 26776682
9. Glycosylation status of nicastrin influences catalytic activity and substrate preference of γ-secretase.  |  Moniruzzaman, M., et al. 2018. Biochem Biophys Res Commun. 502: 98-103. PMID: 29787759
10. Expression analysis elucidates the roles of Nicastrin, Notch4, and Hes1 in prognosis and endocrine-therapy resistance in ER-positive breast cancer patients.  |  Boustan, A., et al. 2023. Res Pharm Sci. 18: 78-88. PMID: 36846736
11. Deficiency of presenilin-1 inhibits the normal cleavage of amyloid precursor protein.  |  De Strooper, B., et al. 1998. Nature. 391: 387-90. PMID: 9450754
12. The presenilin 1 protein is a component of a high molecular weight intracellular complex that contains beta-catenin.  |  Yu, G., et al. 1998. J Biol Chem. 273: 16470-5. PMID: 9632714

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-nicastrin Antibody (B-3) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-nicastrin Antibody (B-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

nicastrin Antibody (B-3) Specifications

Alternate Product Name: NCSTN

Product Clone Name: B-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 23-59

Epitope Target: nicastrin

Epitope Species: human

Product Reactivity/Detection: nicastrin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, bovine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for nicastrin Antibody (B-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin