Nibrin Antibody (A-2): sc-374168
- Nibrin Antibody (A-2) is a mouse monoclonal IgG1 κ, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 1-300 mapping at the N-terminus of Nibrin (NBS1) of human origin
- recommended for detection of Nibrin of human origin by WB, IP, IF, IHC(P) and ELISA
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Nibrin Antibody (A-2) for WB and IHC(P) applications. These reagents are now offered in bundles with Nibrin Antibody (A-2) (see ordering information below).
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Nibrin Antibody (A-2) is a mouse monoclonal IgG1 antibody that detects Nibrin in human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Nibrin plays a crucial role in DNA damage response, particularly in the repair of double-strand breaks, which are among the most lethal forms of DNA damage. Nibrin (A-2) antibody targets proteins primarily located in the nucleus, where Nibrin forms a complex with Mre11 and Rad50, essential components of the MRN complex that senses DNA damage and facilitates repair. Nuclear localization of Nibrin enables rapid recruitment of repair machinery to damage sites, maintaining genomic stability. Mutations or deficiencies in Nibrin are associated with Nijmegen breakage syndrome, a condition characterized by immunodeficiency, increased cancer susceptibility, and sensitivity to ionizing radiation. Anti-Nibrin antibody (A-2) serves as an invaluable tool for researchers studying DNA repair mechanisms and Nibrin′s implications in various diseases, including cancer.
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Nibrin Antibody (A-2) References:
- ATM-dependent phosphorylation of nibrin in response to radiation exposure. | Gatei, M., et al. 2000. Nat Genet. 25: 115-9. PMID: 10802669
- Nijmegen breakage syndrome gene (NBS1) alterations and its protein (nibrin) expression in human ovarian tumours. | Plisiecka-Hałasa, J., et al. 2002. Ann Hum Genet. 66: 353-9. PMID: 12485469
- Roles of nibrin and AtM/ATR kinases on the G2 checkpoint under endogenous or radio-induced DNA damage. | Marcelain, K., et al. 2005. Biol Res. 38: 179-85. PMID: 16238096
- Impact of heterozygous c.657-661del, p.I171V and p.R215W mutations in NBN on nibrin functions. | Dzikiewicz-Krawczyk, A., et al. 2012. Mutagenesis. 27: 337-43. PMID: 22131123
- Cleavage of the BRCT tandem domains of nibrin by the 657del5 mutation affects the DNA damage response less than the Arg215Trp mutation. | Mendez, G., et al. 2012. IUBMB Life. 64: 853-61. PMID: 22941933
- Influence of MRE11, RAD50 and NIBRIN protein expression on survival in pancreatic carcinoma after curative resection. | Horst, K., et al. 2013. Pathol Res Pract. 209: 635-9. PMID: 23954013
- Identification of the interactors of human nibrin (NBN) and of its 26 kDa and 70 kDa fragments arising from the NBN 657del5 founder mutation. | Cilli, D., et al. 2014. PLoS One. 9: e114651. PMID: 25485873
- Deficiency of the DNA repair protein nibrin increases the basal but not the radiation induced mutation frequency in vivo. | Wessendorf, P., et al. 2014. Mutat Res. 769: 11-6. PMID: 25771721
- Decrease of Nibrin expression in chronic hypoxia is associated with hypoxia-induced chemoresistance in some brain tumour cells. | Cowman, S., et al. 2019. BMC Cancer. 19: 300. PMID: 30943920
- Nibrin, a novel DNA double-strand break repair protein, is mutated in Nijmegen breakage syndrome. | Varon, R., et al. 1998. Cell. 93: 467-76. PMID: 9590180
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nibrin Antibody (A-2) | sc-374168 | 200 µg/ml | $322.00 | |||
Nibrin Antibody (A-2): m-IgG Fc BP-HRP Bundle | sc-537924 | 200 µg Ab; 10 µg BP | $361.00 | |||
Nibrin Antibody (A-2): m-IgGκ BP-HRP Bundle | sc-535401 | 200 µg Ab; 40 µg BP | $361.00 | |||
Nibrin Antibody (A-2): m-IgG1 BP-HRP Bundle | sc-545448 | 200 µg Ab; 20 µg BP | $361.00 |