Nibrin Antibody (B-5): sc-515069

Nibrin Antibody (B-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects Nibrin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Nibrin antibody (B-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Nibrin plays a crucial role in DNA damage response, particularly in repairing double-strand breaks, which are among the most lethal forms of DNA damage. Nibrin functions as part of a complex with Mre11 and Rad50, forming the MRE11/RAD50/NBS1 (MRN) complex that is essential for recognizing and processing DNA breaks. Nibrin′s importance is underscored by its absence in Nijmegen breakage syndrome (NBS) patients, who exhibit increased sensitivity to ionizing radiation and predisposition to cancer due to defective DNA repair mechanisms. Nibrin′s interaction with other proteins, such as Rad51 and Rad52, further highlights its integral role in maintaining genomic stability. Rad51 is vital for homologous recombination, while Rad52 facilitates the loading of Rad51 onto DNA, making Nibrin a key player in orchestrating DNA repair processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

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Nibrin Antibody (B-5) References:

1. The common deletion 657del5 in the Nibrin gene is not a major risk factor for B or T cell non-Hodgkin lymphoma in a pediatric population.  |  Rischewski, J., et al. 2000. Leukemia. 14: 1528-9. PMID: 10942254
2. Chromosome instability and nibrin protein variants in NBS heterozygotes.  |  Tanzanella, C., et al. 2003. Eur J Hum Genet. 11: 297-303. PMID: 12708449
3. Identification of a topoisomerase I mutant, scsA1, as an extragenic suppressor of a mutation in scaA(NBS1), the apparent homolog of human nibrin in Aspergillus nidulans.  |  Fagundes, MR., et al. 2003. Genetics. 164: 935-45. PMID: 12871905
4. Cloning of human and mouse genes homologous to RAD52, a yeast gene involved in DNA repair and recombination.  |  Muris, DF., et al. 1994. Mutat Res. 315: 295-305. PMID: 7526206
5. Expression of human RAD52 confers resistance to ionizing radiation in mammalian cells.  |  Park, MS. 1995. J Biol Chem. 270: 15467-70. PMID: 7797537
6. A mouse homolog of the Escherichia coli recA and Saccharomyces cerevisiae RAD51 genes.  |  Morita, T., et al. 1993. Proc Natl Acad Sci U S A. 90: 6577-80. PMID: 8341671
7. Specific interactions between the human RAD51 and RAD52 proteins.  |  Shen, Z., et al. 1996. J Biol Chem. 271: 148-52. PMID: 8550550
8. A mutation in mouse rad51 results in an early embryonic lethal that is suppressed by a mutation in p53.  |  Lim, DS. and Hasty, P. 1996. Mol Cell Biol. 16: 7133-43. PMID: 8943369
9. Nuclear import of DNA repair proteins.  |  Boulikas, T. 1997. Anticancer Res. 17: 843-63. PMID: 9137418
10. Synergistic actions of Rad51 and Rad52 in recombination and DNA repair.  |  Benson, FE., et al. 1998. Nature. 391: 401-4. PMID: 9450758
11. The hMre11/hRad50 protein complex and Nijmegen breakage syndrome: linkage of double-strand break repair to the cellular DNA damage response.  |  Carney, JP., et al. 1998. Cell. 93: 477-86. PMID: 9590181
12. The mouse RecA-like gene Dmc1 is required for homologous chromosome synapsis during meiosis.  |  Yoshida, K., et al. 1998. Mol Cell. 1: 707-18. PMID: 9660954

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Nibrin Antibody (B-5) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-Nibrin Antibody (B-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Nibrin Antibody (B-5) Specifications

Alternate Product Name: NBN

Product Clone Name: B-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 731-754

Epitope Target: Nibrin

Epitope Species: human

Product Reactivity/Detection: Nibrin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for Nibrin Antibody (B-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin