NHE-1 Antibody (B-12): sc-515950
- NHE-1 Antibody (B-12) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 656-815 mapping within a C-terminal cytoplasmic domain of NHE-1 of human origin
- NHE-1 Antibody (B-12) is recommended for detection of NHE-1 of human origin by WB, IP, IF and ELISA
- Anti-NHE-1 Antibody (B-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for NHE-1 Antibody (B-12) for WB applications. These reagents are now offered in bundles with NHE-1 Antibody (B-12) (see ordering information below).
QUICK LINKS
NHE-1 Antibody (B-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects NHE-1 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NHE-1 Antibody (B-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NHE-1, also known as solute carrier family 9 isoform-1 (SLC9A1), plays a crucial role in maintaining cellular homeostasis by regulating intracellular pH and cell volume through the exchange of sodium (Na+) and hydrogen (H+) ions across the plasma membrane. NHE-1 is primarily located in the plasma membrane of various mammalian tissues, where NHE-1 functions are vital for processes such as cell proliferation, migration, and survival, particularly in response to changes in extracellular conditions. NHE-1 is unique among the Na+/H+ exchanger family as NHE-1 is ubiquitously expressed, while other isoforms exhibit more restricted tissue distribution and regulatory mechanisms. The presence of two putative N-glycosylation sites within NHE-1′s structure is significant, as these post-translational modifications can influence stability, localization, and activity, thereby impacting physiological functions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
NHE-1 Antibody (B-12) References:
- Aldosterone increases NHE-1 expression and induces NHE-1-dependent hypertrophy in neonatal rat ventricular myocytes. | Karmazyn, M., et al. 2003. Hypertension. 42: 1171-6. PMID: 14610099
- 3-Substituted-(5-arylfuran-2-ylcarbonyl)guanidines as NHE-1 inhibitors. | Lee, S., et al. 2007. Bioorg Med Chem Lett. 17: 1291-5. PMID: 17189690
- NHE-1: a promising target for novel anti-cancer therapeutics. | Loo, SY., et al. 2012. Curr Pharm Des. 18: 1372-82. PMID: 22360552
- NHE-1 relocation outside cholesterol-rich membrane microdomains is associated with its benzo[a]pyrene-related apoptotic function. | Tekpli, X., et al. 2012. Cell Physiol Biochem. 29: 657-66. PMID: 22613966
- Role of Sodium-Hydrogen Exchanger-1 (NHE-1) in the Effect of Exercise on Intermittent Hypoxia-Induced Left Ventricular Dysfunction. | Chen M, YC., et al. 2015. Chin J Physiol. 58: 254-62. PMID: 26211649
- Inhibition of NHE-1 Increases Smoke-Induced Proliferative Activity of Barrett's Esophageal Cell Line. | Becskeházi, E., et al. 2021. Int J Mol Sci. 22: PMID: 34638919
- Synthesis and multifaceted pharmacological activity of novel quinazoline NHE-1 inhibitors. | Spasov, A., et al. 2021. Sci Rep. 11: 24380. PMID: 34934125
- Guanidine Derivatives of Quinazoline-2,4(1H,3H)-Dione as NHE-1 Inhibitors and Anti-Inflammatory Agents. | Spasov, A., et al. 2022. Life (Basel). 12: PMID: 36295082
- Voltage-Gated Sodium Channel NaV1.5 Controls NHE-1-Dependent Invasive Properties in Colon Cancer Cells. | Lopez-Charcas, O., et al. 2022. Cancers (Basel). 15: PMID: 36612049
- Downregulating NHE-1 decreases the apoptosis of hippocampal cells in epileptic model rats based on the NHE-1/calpain1 pathway. | Peng, S., et al. 2023. Heliyon. 9: e18336. PMID: 37539113
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NHE-1 Antibody (B-12) | sc-515950 | 200 µg/ml | $316.00 | |||
NHE-1 Antibody (B-12): m-IgG Fc BP-HRP Bundle | sc-528071 | 200 µg Ab; 10 µg BP | $354.00 | |||
NHE-1 Antibody (B-12): m-IgG1 BP-HRP Bundle | sc-533444 | 200 µg Ab; 20 µg BP | $354.00 | |||
NHE-1 Antibody (B-12) AC | sc-515950 AC | 500 µg/ml, 25% agarose | $416.00 | |||
NHE-1 Antibody (B-12) HRP | sc-515950 HRP | 200 µg/ml | $316.00 | |||
NHE-1 Antibody (B-12) FITC | sc-515950 FITC | 200 µg/ml | $330.00 | |||
NHE-1 Antibody (B-12) PE | sc-515950 PE | 200 µg/ml | $343.00 | |||
NHE-1 Antibody (B-12) Alexa Fluor® 488 | sc-515950 AF488 | 200 µg/ml | $357.00 | |||
NHE-1 Antibody (B-12) Alexa Fluor® 546 | sc-515950 AF546 | 200 µg/ml | $357.00 | |||
NHE-1 Antibody (B-12) Alexa Fluor® 594 | sc-515950 AF594 | 200 µg/ml | $357.00 | |||
NHE-1 Antibody (B-12) Alexa Fluor® 647 | sc-515950 AF647 | 200 µg/ml | $357.00 | |||
NHE-1 Antibody (B-12) Alexa Fluor® 680 | sc-515950 AF680 | 200 µg/ml | $357.00 | |||
NHE-1 Antibody (B-12) Alexa Fluor® 790 | sc-515950 AF790 | 200 µg/ml | $357.00 |