NF-M Antibody (NF-09): sc-51683

NF-M Antibody (NF-09) is a mouse monoclonal IgG2a antibody that detects neurofilament-M (NF-M) in mouse, rat, human, and porcine samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. NF-M is a crucial component of the neuronal cytoskeleton, belonging to the intermediate filament family, and plays a significant role in maintaining the structural integrity of neurons. One of the key aspects of NF-M is its phosphorylation, which is regulated by various protein kinases, including protein kinase A and cyclin-dependent kinase 5. This post-translational modification is vital as it influences the dynamics of neurofilament assembly and disassembly, thereby affecting axon caliber and overall neuronal function. Dysregulation of NF-M phosphorylation has been implicated in several neurodegenerative diseases, such as amyotrophic lateral sclerosis, Parkinson′s disease, and Alzheimer′s disease, highlighting the importance of NF-M in both healthy and diseased states. Through interactions with other cytoskeletal elements, NF-M contributes to axon stability and participates in intricate signaling pathways governing neuronal health. Anti-NF-M antibody (NF-09) serves as an essential tool for researchers investigating neurofilament dynamics and their implications in neurodegenerative conditions.

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NF-M Antibody (NF-09) References:

1. RFLP for BgI II at the human neurofilament medium chain (NEF3) gene locus.  |  Ding, Y., et al. 1992. Nucleic Acids Res. 20: 1429. PMID: 1348579
2. Metal ion-induced conformational changes of phosphorylated fragments of human neurofilament (NF-M) protein.  |  Hollósi, M., et al. 1992. J Mol Biol. 223: 673-82. PMID: 1542114
3. Characterization of the bovine neurofilament NF-M protein and cDNA sequence, and identification of in vitro and in vivo calpain cleavage sites.  |  Shaw, G., et al. 2004. Biochem Biophys Res Commun. 325: 619-25. PMID: 15530438
4. Phosphorylation of neurofilament subunit NF-M is regulated by activation of NMDA receptors and modulates cytoskeleton stability and neuronal shape.  |  Fiumelli, H., et al. 2008. Cell Motil Cytoskeleton. 65: 495-504. PMID: 18412220
5. Characterization of dominant and recessive assembly-defective mutations in mouse neurofilament NF-M.  |  Wong, PC. and Cleveland, DW. 1990. J Cell Biol. 111: 1987-2003. PMID: 2121743
6. The C-terminal domains of NF-H and NF-M subunits maintain axonal neurofilament content by blocking turnover of the stationary neurofilament network.  |  Rao, MV., et al. 2012. PLoS One. 7: e44320. PMID: 23028520
7. Expression of NF-L and NF-M in fibroblasts reveals coassembly of neurofilament and vimentin subunits.  |  Monteiro, MJ. and Cleveland, DW. 1989. J Cell Biol. 108: 579-93. PMID: 2493000
8. Monoclonal antibodies distinguish several differentially phosphorylated states of the two largest rat neurofilament subunits (NF-H and NF-M) and demonstrate their existence in the normal nervous system of adult rats.  |  Lee, VM., et al. 1987. J Neurosci. 7: 3474-88. PMID: 3119789
9. Phosphorylation of native and reassembled neurofilaments composed of NF-L, NF-M, and NF-H by the catalytic subunit of cAMP-dependent protein kinase.  |  Hisanaga, S., et al. 1994. Mol Biol Cell. 5: 161-72. PMID: 8019002
10. Transient expression of the neurofilament proteins NF-L and NF-M by Schwann cells is regulated by axonal contact.  |  Fabrizi, C., et al. 1997. J Neurosci Res. 50: 291-9. PMID: 9373038

The preferred NF-M antibody is NF-M Antibody (E-9): sc-398532.