NET1 Antibody (G-4): sc-271941

NET1 Antibody (G-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects NET1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-NET1 antibody (G-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NET1 plays a crucial role in regulating the actin cytoskeleton and is involved in various cellular processes, including cell migration and invasion, which are vital for normal physiological functions and pathological conditions such as cancer metastasis. Specifically, NET1 acts as a guanine nucleotide exchange factor (GEF) for RhoA and Cdc42, facilitating the exchange of GDP for GTP, thereby activating these small GTPases that are essential for cytoskeletal dynamics. NET1′s importance in cellular signaling pathways is underscored by involvement in oncogenic processes; overexpression of NET1 has been linked to altered growth properties and tumorigenesis in NIH/3T3 cells, highlighting potential as a target for therapeutic intervention in cancer. NET1 (G-4) antibody enables researchers to explore NET1′s intricate roles in various biological contexts, making this tool valuable for advancing understanding of GTPase signaling and implications in health and disease.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

NET1 Antibody (G-4) References:

1. Development of a highly efficient expression cDNA cloning system: application to oncogene isolation.  |  Miki, T., et al. 1991. Proc Natl Acad Sci U S A. 88: 5167-71. PMID: 2052597
2. A region of proto-dbl essential for its transforming activity shows sequence similarity to a yeast cell cycle gene, CDC24, and the human breakpoint cluster gene, bcr.  |  Ron, D., et al. 1991. New Biol. 3: 372-9. PMID: 2065022
3. A functional and transcriptomic analysis of NET1 bioactivity in gastric cancer.  |  Bennett, G., et al. 2011. BMC Cancer. 11: 50. PMID: 21284875
4. NET1 Enhances Proliferation and Chemoresistance in Acute Lymphoblastic Leukemia Cells.  |  Sun, H., et al. 2019. Oncol Res. 27: 935-944. PMID: 31046876
5. Simultaneous binding of Guidance Cues NET1 and RGM blocks extracellular NEO1 signaling.  |  Robinson, RA., et al. 2021. Cell. 184: 2103-2120.e31. PMID: 33740419
6. NET1 is a critical regulator of spindle assembly and actin dynamics in mouse oocytes.  |  Wang, S., et al. 2024. Reprod Biol Endocrinol. 22: 5. PMID: 38169395
7. GTPase cascades choreographing cellular behavior: movement, morphogenesis, and more.  |  Chant, J. and Stowers, L. 1995. Cell. 81: 1-4. PMID: 7720065
8. A novel oncogene, ost, encodes a guanine nucleotide exchange factor that potentially links Rho and Rac signaling pathways.  |  Horii, Y., et al. 1994. EMBO J. 13: 4776-86. PMID: 7957046
9. Proteins regulating Ras and its relatives.  |  Boguski, MS. and McCormick, F. 1993. Nature. 366: 643-54. PMID: 8259209
10. Cellular transformation and guanine nucleotide exchange activity are catalyzed by a common domain on the dbl oncogene product.  |  Hart, MJ., et al. 1994. J Biol Chem. 269: 62-5. PMID: 8276860
11. A putative modular domain present in diverse signaling proteins.  |  Mayer, BJ., et al. 1993. Cell. 73: 629-30. PMID: 8500161
12. Isolation of a novel oncogene, NET1, from neuroepithelioma cells by expression cDNA cloning.  |  Chan, AM., et al. 1996. Oncogene. 12: 1259-66. PMID: 8649828

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NET1 Antibody (G-4) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-NET1 Antibody (G-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NET1 Antibody (G-4) Specifications

Alternate Product Name: NET1

Product Clone Name: G-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 106-175

Epitope Target: NET1

Epitope Species: human

Product Reactivity/Detection: Rho-specific GEF NET1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for NET1 Antibody (G-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin