nestin Antibody (D-9) is a mouse monoclonal IgG2a κ, cited in 3 publications, provided at 200 µg/ml
raised against amino acids 235-320 of nestin of human origin
Anti-nestin Antibody (D-9) is recommended for detection of nestin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
Anti-nestin Antibody (D-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of nestin (D-9): sc-377380.
At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for nestin Antibody (D-9). This work is in progress.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
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Labware ships FedEx Ground free of charge to the contiguous US
Nestin is a major intermediate filament (IF) protein of embryonic central nervous system progenitor cells. It is also a component of the dynamic IF network during muscle development, where it polymerizes with Desmin and Vimentin. Nestin co-assembles with Vimentin or a-internexin and forms heterodimer coiled-coil molecules which then further assemble into 10 nml IFs. Deletion of the IF consensus rod domain in nestin alters nestin localization in CNS precursor cells and radial glial cells in vivo. Nestin is a marker for neuroepithelial stem cells, glioma cells and tumor endothelial cells during rapid growth. During axon elongation of differen-tiation neurons, nestin localizes to the growth cones and may play a role in growth cone guidance. In the rat adrenal gland, nestin is expressed by the zona fasciculata and the zona reticularis. Nestin is also expressed by dermatomal cells and by myoblasts during the earliest stages of myogenesis.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Rated 5 out of
Great antibodyI've used this antibody for WB and it worked very good. I recommend it
Date published: 2018-01-08
Rated 5 out of
Beautiful IHC(P) resultsThis nestin antibody gives beautiful staining data in formalin fixed, paraffin-embedded human kidney tissue showing membrane staining of cells in glomeruli.
Date published: 2017-02-03
Rated 4 out of
Positive signal achieved in IMRPositive signal achieved in IMR-32 whole cell lysate. -SCBT QC
Date published: 2015-03-07
Rated 5 out of
Produced excellent immunoperoxidase membraneProduced excellent immunoperoxidase membrane staining in formalin fixed, paraffin-embedded human kidney tissue. -SCBT QC
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