nestin Antibody (10c2): sc-23927

Nestin Antibody (10c2) is a mouse monoclonal IgG1 kappa light chain antibody that detects nestin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Anti-nestin antibody (10c2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Nestin is a crucial intermediate filament protein predominantly found in embryonic central nervous system progenitor cells, playing a vital role in structural integrity and function. Nestin expression is essential during muscle development, where nestin interacts with other intermediate filament proteins such as Desmin and Vimentin, forming a dynamic network that supports cellular architecture. Nestin′s ability to co-assemble with Vimentin or α-internexin into heterodimer coiled-coil structures leads to the formation of 10 nm intermediate filaments critical for maintaining cellular shape and resilience. Deletion of nestin in the intermediate filament consensus rod domain can disrupt nestin localization in central nervous system precursor cells and radial glial cells, which may affect neurodevelopmental processes. Nestin serves as a marker for neuroepithelial stem cells, glioma cells, and tumor endothelial cells during periods of rapid growth, highlighting nestin′s importance in both normal development and pathological conditions. During neuronal differentiation, nestin localizes to growth cones, suggesting a role in guiding axon elongation, which is crucial for proper neural network formation. Nestin expression is observed in the zona fasciculata and zona reticularis of rat adrenal gland, as well as in dermatomal cells and myoblasts during early stages of myogenesis, further underscoring nestin′s diverse roles in various tissues.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

nestin Antibody (10c2) References:

1. A high molecular weight intermediate filament-associated protein in BHK-21 cells is nestin, a type VI intermediate filament protein. Limited co-assembly in vitro to form heteropolymers with type III vimentin and type IV alpha-internexin.  |  Steinert, PM., et al. 1999. J Biol Chem. 274: 9881-90. PMID: 10092680
2. Mouse nestin protein localizes in growth cones of P19 neurons and cerebellar granule cells.  |  Yan, Y., et al. 2001. Neurosci Lett. 302: 89-92. PMID: 11290394
3. Nestin as a marker for proliferative endothelium in gliomas.  |  Sugawara, K., et al. 2002. Lab Invest. 82: 345-51. PMID: 11896213
4. Nestin expression in rat adrenal gland.  |  Bertelli, E., et al. 2002. Histochem Cell Biol. 117: 371-7. PMID: 11976910
5. CNS stem cells express a new class of intermediate filament protein.  |  Lendahl, U., et al. 1990. Cell. 60: 585-95. PMID: 1689217
6. Myogenesis and the intermediate filament protein, nestin.  |  Kachinsky, AM., et al. 1994. Dev Biol. 165: 216-28. PMID: 8088440
7. Transient expression of the intermediate filament nestin during skeletal muscle development.  |  Sejersen, T. and Lendahl, U. 1993. J Cell Sci. 106 (Pt 4): 1291-300. PMID: 8126108
8. A rod end deletion in the intermediate filament protein nestin alters its subcellular localization in neuroepithelial cells of transgenic mice.  |  Marvin, MJ., et al. 1998. J Cell Sci. 111 (Pt 14): 1951-61. PMID: 9645943

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-nestin Antibody (10c2) has 239 citations in a variety of scientific publications.

Primary antibodies like Anti-nestin Antibody (10c2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

nestin Antibody (10c2) Specifications

Alternate Product Name: nestin

Product Clone Name: 10c2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: nestin

Epitope Species: human

Product Reactivity/Detection: nestin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 239 citations in various scientific publications

Available Conjugates/Forms for nestin Antibody (10c2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin