Anti-NEPH1 Antibody (F-6) is a mouse monoclonal IgG2a (kappa light chain) NEPH1 antibody provided at 200 µg/ml
raised against amino acids 608-757 mapping within a C-terminal cytoplasmic domain of NEPH1 of human origin
Anti-NEPH1 Antibody (F-6) is recommended for detection of NEPH1 of mouse, rat and human origin by WB, IP, IF and ELISA
Anti-NEPH1 Antibody (F-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of NEPH1 (F-6): sc-373787.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
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Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
NEPH1 Antibody (F-6) is a high quality monoclonal NEPH1 antibody (also designated NEPH1 antibody) suitable for the detection of the NEPH1 protein of mouse, rat and human origin. NEPH1 Antibody (F-6) is available as both the non-conjugated anti-NEPH1 antibody form, as well as multiple conjugated forms of anti-NEPH1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Glomerular visceral epithelial cells, also known as podocytes, maintain the selective filtration barrier of the renal glomerulus. NEPH1, a member of the immunoglobulin superfamily, plays a critical role in functional barrier development. Loss of NEPH1 expression, like that of its structural relative nephrin, results in nephrotic syndromes and proteinuria leading to perinatal death. NEPH1 associates with nephrin as well as ZO-1 and localizes with them to the glomerular slit diaphragm. Interaction with nephrin occurs via the extracellular domain of NEPH1 and with ZO-1 in a PDZ binding motif of the cytoplasmic tail. Mutation of a putative threonine phosphorylation site within the cytoplasmic domain abrogates interaction with ZO-1, implying that phosphorylation regulates this interaction, and may effect the recruitment of the appropriate signal transduction components to the complex.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences