NELF-A Antibody (F-2): sc-271731

NELF-A Antibody (F-2) is a mouse monoclonal IgG1 antibody that detects NELF-A in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NELF-A, also known as WHSC2 (Wolf-Hirschhorn syndrome candidate 2), plays a crucial role in transcription elongation regulation by forming part of the multisubunit NELF complex, which works with DSIF to inhibit RNA polymerase II elongation. This regulation ensures precisely controlled transcription, allowing cells to respond appropriately to various signals. The NELF complex, including NELF-A, maintains the balance between transcriptional activation and repression, particularly important in development and differentiation. The human NELF-A gene is located on chromosome 4p16.3, and hemizygous deletion is associated with Wolf-Hirschhorn syndrome, a condition characterized by significant mental and developmental challenges. NELF-A is expressed in endothelial cells and consists of 528 amino acids, highlighting importance in cellular function and development. anti-NELF-A antibody (F-2) is an invaluable tool for researchers studying transcription regulation and implications in various biological processes and diseases.

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NELF-A Antibody (F-2) References:

1. NELF, a multisubunit complex containing RD, cooperates with DSIF to repress RNA polymerase II elongation.  |  Yamaguchi, Y., et al. 1999. Cell. 97: 41-51. PMID: 10199401
2. Human transcription elongation factor NELF: identification of novel subunits and reconstitution of the functionally active complex.  |  Narita, T., et al. 2003. Mol Cell Biol. 23: 1863-73. PMID: 12612062
3. Negative elongation factor NELF represses human immunodeficiency virus transcription by pausing the RNA polymerase II complex.  |  Zhang, Z., et al. 2007. J Biol Chem. 282: 16981-8. PMID: 17442680
4. Nasal embryonic LHRH factor (NELF) mutations in patients with normosmic hypogonadotropic hypogonadism and Kallmann syndrome.  |  Xu, N., et al. 2011. Fertil Steril. 95: 1613-20.e1-7. PMID: 21300340
5. Characterizing the functional consequences of haploinsufficiency of NELF-A (WHSC2) and SLBP identifies novel cellular phenotypes in Wolf-Hirschhorn syndrome.  |  Kerzendorfer, C., et al. 2012. Hum Mol Genet. 21: 2181-93. PMID: 22328085
6. A conserved protein motif is required for full modulatory activity of negative elongation factor subunits NELF-A and NELF-B in modifying glucocorticoid receptor-regulated gene induction properties.  |  Luo, M., et al. 2013. J Biol Chem. 288: 34055-34072. PMID: 24097989
7. NELF-E is recruited to DNA double-strand break sites to promote transcriptional repression and repair.  |  Awwad, SW., et al. 2017. EMBO Rep. 18: 745-764. PMID: 28336775
8. NELF complex fosters BRCA1 and RAD51 recruitment to DNA damage sites and modulates sensitivity to PARP inhibition.  |  Bishara, LA., et al. 2021. DNA Repair (Amst). 97: 103025. PMID: 33248388
9. ERK-mediated NELF-A phosphorylation promotes transcription elongation of immediate-early genes by releasing promoter-proximal pausing of RNA polymerase II.  |  Ohe, S., et al. 2022. Nat Commun. 13: 7476. PMID: 36463234
10. Regulation of aging by NELF-A and RNA polymerase II elongation.  |  Ong, CT. and Ngian, ZK. 2024. Neural Regen Res. 19: 1415-1416. PMID: 38051874

The preferred NELF-A antibody is NELF-A Antibody (G-11): sc-365004.