NEDD1 Antibody (H-3): sc-398733
- NEDD1 Antibody (H-3) is a mouse monoclonal IgG1 κ NEDD1 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 61-360 mapping near the N-terminus of NEDD1 of human origin
- NEDD1 Antibody (H-3) is recommended for detection of NEDD1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-NEDD1 Antibody (H-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for NEDD1 Antibody (H-3) for WB applications. These reagents are now offered in bundles with NEDD1 Antibody (H-3) (see ordering information below).
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NEDD1 Antibody (H-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects NEDD1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-NEDD1 antibody (H-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NEDD1, also known as neural precursor cell expressed, developmentally down-regulated 1 or GCP-WD, is a highly conserved protein that plays a pivotal role in microtubule nucleation during mitosis. NEDD1 is characterized by the presence of eight WD40 repeats and a coiled-coil domain at its C-terminus, which are essential for function in the γ-tubulin ring complex (γTuRC). Post-translational modifications, particularly phosphorylation, regulate NEDD1 localization and activity, which is crucial for interaction with γ-tubulin and targeting to the centrosome and spindle apparatus. This regulation is vital for proper microtubule organization and function during cell division. Disruption of NEDD1 function can lead to impaired microtubule nucleation and has been implicated in various cancerous processes, making NEDD1 a potential target for therapeutic intervention in cancer treatment.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
NEDD1 Antibody (H-3) References:
- Characteristic promoter hypermethylation signatures in male germ cell tumors. | Koul, S., et al. 2002. Mol Cancer. 1: 8. PMID: 12495446
- GCP-WD is a gamma-tubulin targeting factor required for centrosomal and chromatin-mediated microtubule nucleation. | Lüders, J., et al. 2006. Nat Cell Biol. 8: 137-47. PMID: 16378099
- NEDD1-dependent recruitment of the gamma-tubulin ring complex to the centrosome is necessary for centriole duplication and spindle assembly. | Haren, L., et al. 2006. J Cell Biol. 172: 505-15. PMID: 16461362
- NEDD1: function in microtubule nucleation, spindle assembly and beyond. | Manning, J. and Kumar, S. 2007. Int J Biochem Cell Biol. 39: 7-11. PMID: 17005434
- [NEDD1 and microtubule nucleation complexes: To recruit for better organizing]. | Haren, L., et al. 2006. Med Sci (Paris). 22: 804-6. PMID: 17026925
- Microtubule-dependent microtubule nucleation in plant cells. | Murata, T. and Hasebe, M. 2007. J Plant Res. 120: 73-8. PMID: 17160422
- Assignment of the developmentally regulated gene NEDD1 to human chromosome 12q22 by fluorescence in situ hybridization. | Takai, S., et al. 1995. Hum Genet. 95: 96-8. PMID: 7814034
- Induction of apoptosis by the mouse Nedd2 gene, which encodes a protein similar to the product of the Caenorhabditis elegans cell death gene ced-3 and the mammalian IL-1 beta-converting enzyme. | Kumar, S., et al. 1994. Genes Dev. 8: 1613-26. PMID: 7958843
- Molecular cloning and biological activity of a novel developmentally regulated gene encoding a protein with beta-transducin-like structure. | Kumar, S., et al. 1994. J Biol Chem. 269: 11318-26. PMID: 8157664
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NEDD1 Antibody (H-3) | sc-398733 | 200 µg/ml | $316.00 | |||
NEDD1 Antibody (H-3): m-IgG Fc BP-HRP Bundle | sc-530907 | 200 µg Ab; 10 µg BP | $354.00 | |||
NEDD1 Antibody (H-3): m-IgGκ BP-HRP Bundle | sc-524461 | 200 µg Ab, 40 µg BP | $354.00 | |||
NEDD1 Antibody (H-3): m-IgG1 BP-HRP Bundle | sc-544313 | 200 µg Ab; 20 µg BP | $354.00 | |||
NEDD1 Antibody (H-3) AC | sc-398733 AC | 500 µg/ml, 25% agarose | $416.00 | |||
NEDD1 Antibody (H-3) HRP | sc-398733 HRP | 200 µg/ml | $316.00 | |||
NEDD1 Antibody (H-3) FITC | sc-398733 FITC | 200 µg/ml | $330.00 | |||
NEDD1 Antibody (H-3) PE | sc-398733 PE | 200 µg/ml | $343.00 | |||
NEDD1 Antibody (H-3) Alexa Fluor® 488 | sc-398733 AF488 | 200 µg/ml | $357.00 | |||
NEDD1 Antibody (H-3) Alexa Fluor® 546 | sc-398733 AF546 | 200 µg/ml | $357.00 | |||
NEDD1 Antibody (H-3) Alexa Fluor® 594 | sc-398733 AF594 | 200 µg/ml | $357.00 | |||
NEDD1 Antibody (H-3) Alexa Fluor® 647 | sc-398733 AF647 | 200 µg/ml | $357.00 | |||
NEDD1 Antibody (H-3) Alexa Fluor® 680 | sc-398733 AF680 | 200 µg/ml | $357.00 | |||
NEDD1 Antibody (H-3) Alexa Fluor® 790 | sc-398733 AF790 | 200 µg/ml | $357.00 |