Nectin 1 Antibody (CK6): sc-21722
- Nectin 1 Antibody (CK6) is a mouse monoclonal IgG1 κ Nectin 1 antibody, cited in 22 publications, provided at 200 µg/ml
- raised against the V-domain of Nectin 1
- Nectin 1 Antibody (CK6) is recommended for detection of Nectin 1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
- Anti-Nectin 1 Antibody (CK6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Nectin 1 Antibody (CK6) for WB and IHC(P) applications. These reagents are now offered in bundles with Nectin 1 Antibody (CK6) (see ordering information below).
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Nectin 1 Antibody (CK6) is a mouse monoclonal IgG1 kappa light chain antibody that detects Nectin 1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). Anti-Nectin 1 antibody (CK6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Nectin 1 is a crucial Ca2+-independent homophilic cell adhesion molecule that plays a significant role in cellular interactions and signaling, belonging to the immunoglobulin superfamily. Nectin 1 serves as a receptor for herpes simplex virus types 1 and 2, facilitating viral entry into host cells. Nectin 1 is expressed abundantly in the brain and exists in two isoforms, Nectin 1α and Nectin 1β/HIgR, produced through alternative splicing. The cytoplasmic region of Nectin 1α contains a conserved C-terminal motif that interacts with the PDZ domain of afadin, linking Nectin 1 to the actin cytoskeleton and playing a vital role in maintaining cell adhesion and integrity at adherens junctions. The interaction between Nectin 1 and viral glycoprotein D is essential for membrane fusion during viral entry, highlighting Nectin 1′s significance in both cell biology and virology. Nectin 1 (CK6) monoclonal antibody is an invaluable tool for researchers studying cell adhesion mechanisms, viral pathogenesis, and molecular interactions that underpin cellular architecture.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Nectin 1 Antibody (CK6) References:
- Cell-cell adhesion-mediated tyrosine phosphorylation of nectin-2delta, an immunoglobulin-like cell adhesion molecule at adherens junctions. | Kikyo, M., et al. 2000. Oncogene. 19: 4022-8. PMID: 10962558
- Two cell adhesion molecules, nectin and cadherin, interact through their cytoplasmic domain-associated proteins. | Tachibana, K., et al. 2000. J Cell Biol. 150: 1161-76. PMID: 10974003
- Localization of a binding site for herpes simplex virus glycoprotein D on herpesvirus entry mediator C by using antireceptor monoclonal antibodies. | Krummenacher, C., et al. 2000. J Virol. 74: 10863-72. PMID: 11069980
- Striking similarity of murine nectin-1alpha to human nectin-1alpha (HveC) in sequence and activity as a glycoprotein D receptor for alphaherpesvirus entry. | Shukla, D., et al. 2000. J Virol. 74: 11773-81. PMID: 11090177
- Nectin-1 and HVEM: cellular receptors for HSV-1 in skin. | Knebel-Mörsdorf, D. 2016. Oncotarget. 7: 19087-8. PMID: 27031306
- Nectin-1 Is an Entry Mediator for Varicella-Zoster Virus Infection of Human Neurons. | Rajbhandari, L., et al. 2021. J Virol. 95: e0122721. PMID: 34468169
- Nectin-1 and Non-muscle Myosin Heavy Chain-IIB: Major Mediators of Herpes Simplex Virus-1 Entry Into Corneal Nerves. | Wang, C., et al. 2022. Front Microbiol. 13: 830699. PMID: 35295302
- High expression of nectin-1 indicates a poor prognosis and promotes metastasis in hepatocellular carcinoma. | Wang, X., et al. 2022. Front Oncol. 12: 953529. PMID: 36059705
- Cell entry of Bovine herpesvirus-1 through clathrin- and caveolin-mediated endocytosis requires activation of PI3K-Akt-NF-κB and Ras-p38 MAPK pathways as well as the interaction of BoHV-1 gD with cellular receptor nectin-1. | Liu, Y., et al. 2023. Vet Microbiol. 279: 109672. PMID: 36774841
- Ex Vivo Infection of Human Skin Models with Herpes Simplex Virus 1: Accessibility of the Receptor Nectin-1 during Formation or Impairment of Epidermal Barriers Is Restricted by Tight Junctions. | De La Cruz, NC., et al. 2023. J Virol. 97: e0026223. PMID: 37289055
- Complementary DNA characterization and chromosomal localization of a human gene related to the poliovirus receptor-encoding gene. | Lopez, M., et al. 1995. Gene. 155: 261-5. PMID: 7721102
- Entry of alphaherpesviruses mediated by poliovirus receptor-related protein 1 and poliovirus receptor. | Geraghty, RJ., et al. 1998. Science. 280: 1618-20. PMID: 9616127
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nectin 1 Antibody (CK6) | sc-21722 | 200 µg/ml | $322.00 | |||
Nectin 1 Antibody (CK6): m-IgG Fc BP-HRP Bundle | sc-528363 | 200 µg Ab; 10 µg BP | $361.00 | |||
Nectin 1 Antibody (CK6): m-IgGκ BP-HRP Bundle | sc-520724 | 200 µg Ab, 40 µg BP | $361.00 | |||
Nectin 1 Antibody (CK6): m-IgG1 BP-HRP Bundle | sc-542892 | 200 µg Ab; 20 µg BP | $361.00 | |||
Nectin 1 Antibody (CK6) AC | sc-21722 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Nectin 1 Antibody (CK6) HRP | sc-21722 HRP | 200 µg/ml | $322.00 | |||
Nectin 1 Antibody (CK6) FITC | sc-21722 FITC | 200 µg/ml | $336.00 | |||
Nectin 1 Antibody (CK6) PE | sc-21722 PE | 200 µg/ml | $349.00 | |||
Nectin 1 Antibody (CK6) Alexa Fluor® 488 | sc-21722 AF488 | 200 µg/ml | $364.00 | |||
Nectin 1 Antibody (CK6) Alexa Fluor® 546 | sc-21722 AF546 | 200 µg/ml | $364.00 | |||
Nectin 1 Antibody (CK6) Alexa Fluor® 594 | sc-21722 AF594 | 200 µg/ml | $364.00 | |||
Nectin 1 Antibody (CK6) Alexa Fluor® 647 | sc-21722 AF647 | 200 µg/ml | $364.00 | |||
Nectin 1 Antibody (CK6) Alexa Fluor® 680 | sc-21722 AF680 | 200 µg/ml | $364.00 | |||
Nectin 1 Antibody (CK6) Alexa Fluor® 790 | sc-21722 AF790 | 200 µg/ml | $364.00 |