Nectin 1 Antibody (CK6): sc-21722

Nectin 1 Antibody (CK6) is a mouse monoclonal IgG1 kappa light chain antibody that detects Nectin 1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). Anti-Nectin 1 antibody (CK6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Nectin 1 is a crucial Ca2+-independent homophilic cell adhesion molecule that plays a significant role in cellular interactions and signaling, belonging to the immunoglobulin superfamily. Nectin 1 serves as a receptor for herpes simplex virus types 1 and 2, facilitating viral entry into host cells. Nectin 1 is expressed abundantly in the brain and exists in two isoforms, Nectin 1α and Nectin 1β/HIgR, produced through alternative splicing. The cytoplasmic region of Nectin 1α contains a conserved C-terminal motif that interacts with the PDZ domain of afadin, linking Nectin 1 to the actin cytoskeleton and playing a vital role in maintaining cell adhesion and integrity at adherens junctions. The interaction between Nectin 1 and viral glycoprotein D is essential for membrane fusion during viral entry, highlighting Nectin 1′s significance in both cell biology and virology. Nectin 1 (CK6) monoclonal antibody is an invaluable tool for researchers studying cell adhesion mechanisms, viral pathogenesis, and molecular interactions that underpin cellular architecture.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Nectin 1 Antibody (CK6) References:

1. Cell-cell adhesion-mediated tyrosine phosphorylation of nectin-2delta, an immunoglobulin-like cell adhesion molecule at adherens junctions.  |  Kikyo, M., et al. 2000. Oncogene. 19: 4022-8. PMID: 10962558
2. Two cell adhesion molecules, nectin and cadherin, interact through their cytoplasmic domain-associated proteins.  |  Tachibana, K., et al. 2000. J Cell Biol. 150: 1161-76. PMID: 10974003
3. Localization of a binding site for herpes simplex virus glycoprotein D on herpesvirus entry mediator C by using antireceptor monoclonal antibodies.  |  Krummenacher, C., et al. 2000. J Virol. 74: 10863-72. PMID: 11069980
4. Striking similarity of murine nectin-1alpha to human nectin-1alpha (HveC) in sequence and activity as a glycoprotein D receptor for alphaherpesvirus entry.  |  Shukla, D., et al. 2000. J Virol. 74: 11773-81. PMID: 11090177
5. Nectin-1 and HVEM: cellular receptors for HSV-1 in skin.  |  Knebel-Mörsdorf, D. 2016. Oncotarget. 7: 19087-8. PMID: 27031306
6. Nectin-1 Is an Entry Mediator for Varicella-Zoster Virus Infection of Human Neurons.  |  Rajbhandari, L., et al. 2021. J Virol. 95: e0122721. PMID: 34468169
7. Nectin-1 and Non-muscle Myosin Heavy Chain-IIB: Major Mediators of Herpes Simplex Virus-1 Entry Into Corneal Nerves.  |  Wang, C., et al. 2022. Front Microbiol. 13: 830699. PMID: 35295302
8. High expression of nectin-1 indicates a poor prognosis and promotes metastasis in hepatocellular carcinoma.  |  Wang, X., et al. 2022. Front Oncol. 12: 953529. PMID: 36059705
9. Cell entry of Bovine herpesvirus-1 through clathrin- and caveolin-mediated endocytosis requires activation of PI3K-Akt-NF-κB and Ras-p38 MAPK pathways as well as the interaction of BoHV-1 gD with cellular receptor nectin-1.  |  Liu, Y., et al. 2023. Vet Microbiol. 279: 109672. PMID: 36774841
10. Ex Vivo Infection of Human Skin Models with Herpes Simplex Virus 1: Accessibility of the Receptor Nectin-1 during Formation or Impairment of Epidermal Barriers Is Restricted by Tight Junctions.  |  De La Cruz, NC., et al. 2023. J Virol. 97: e0026223. PMID: 37289055
11. Complementary DNA characterization and chromosomal localization of a human gene related to the poliovirus receptor-encoding gene.  |  Lopez, M., et al. 1995. Gene. 155: 261-5. PMID: 7721102
12. Entry of alphaherpesviruses mediated by poliovirus receptor-related protein 1 and poliovirus receptor.  |  Geraghty, RJ., et al. 1998. Science. 280: 1618-20. PMID: 9616127

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Nectin 1 Antibody (CK6) has 22 citations in a variety of scientific publications.

Primary antibodies like Anti-Nectin 1 Antibody (CK6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Nectin 1 Antibody (CK6) Specifications

Alternate Product Name: Nectin 1

Product Clone Name: CK6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Nectin 1

Epitope Species: human

Product Reactivity/Detection: Nectin 1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 22 citations in various scientific publications

Available Conjugates/Forms for Nectin 1 Antibody (CK6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin