NDUFS1 Antibody (E-8): sc-271510

NDUFS1 Antibody (E-8) is a mouse monoclonal IgG2b kappa light chain antibody that detects NDUFS1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). NDUFS1 (E-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NDUFS1, a 75 kDa subunit of the NADH:ubiquinone oxidoreductase complex (complex I), plays a pivotal role in the mitochondrial electron transport chain, which is essential for ATP production through oxidative phosphorylation. NDUFS1 is crucial for transferring electrons from NADH to the electron transport chain and serves as the first iron-sulfur protein to accept electrons from NADH-flavoprotein reductase within the complex. This function maintains cellular energy levels and overall metabolic health. NDUFS1 is also implicated in apoptosis, serving as a critical substrate for caspases in mitochondria, with cleavage necessary for mitochondrial changes associated with programmed cell death. NDUFS1′s interactions with other proteins within the electron transport chain underscore its importance in cellular respiration and energy metabolism, making anti-NDUFS1 antibody (E-8) an invaluable tool for researchers studying mitochondrial function and related pathologies.

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NDUFS1 Antibody (E-8) References:

1. Large-scale deletion and point mutations of the nuclear NDUFV1 and NDUFS1 genes in mitochondrial complex I deficiency.  |  Bénit, P., et al. 2001. Am J Hum Genet. 68: 1344-52. PMID: 11349233
2. Localization of the human 75-kDal Fe-S protein of NADH-coenzyme Q reductase gene (NDUFS1) to 2q33----q34.  |  Duncan, AM., et al. 1992. Cytogenet Cell Genet. 60: 212-3. PMID: 1505218
3. Leigh syndrome associated with mitochondrial complex I deficiency due to a novel mutation in the NDUFS1 gene.  |  Martín, MA., et al. 2005. Arch Neurol. 62: 659-61. PMID: 15824269
4. A high-fat diet coordinately downregulates genes required for mitochondrial oxidative phosphorylation in skeletal muscle.  |  Sparks, LM., et al. 2005. Diabetes. 54: 1926-33. PMID: 15983191
5. Ultrasound evaluation of peritoneal catheter tunnel in catheter related infections in CAPD.  |  Karahan, OI., et al. 2005. Int Urol Nephrol. 37: 363-6. PMID: 16142572
6. Cell biological consequences of mitochondrial NADH: ubiquinone oxidoreductase deficiency.  |  Smeitink, JA., et al. 2004. Curr Neurovasc Res. 1: 29-40. PMID: 16181064
7. A possible role for iron-sulfur cluster N2 in proton translocation by the NADH: ubiquinone oxidoreductase (complex I).  |  Flemming, D., et al. 2005. J Mol Microbiol Biotechnol. 10: 208-22. PMID: 16645316
8. Topography and chemical reactivity of the active-inactive transition-sensitive SH-group in the mitochondrial NADH:ubiquinone oxidoreductase (Complex I).  |  Gostimskaya, IS., et al. 2006. Biochim Biophys Acta. 1757: 1155-61. PMID: 16777054
9. Determination of the cDNA sequence for the human mitochondrial 75-kDa Fe-S protein of NADH-coenzyme Q reductase.  |  Chow, W., et al. 1991. Eur J Biochem. 201: 547-50. PMID: 1935949
10. MiR-3130-5p is an intermediate modulator of 2q33 and influences the invasiveness of lung adenocarcinoma by targeting NDUFS1.  |  Zhan, J., et al. 2021. Cancer Med. 10: 3700-3714. PMID: 33978320
11. Proteomic Analysis Identifies NDUFS1 and ATP5O as Novel Markers for Survival Outcome in Prostate Cancer.  |  Wiebringhaus, R., et al. 2021. Cancers (Basel). 13: PMID: 34885151
12. Conditions Conducive to the Glutathionylation of Complex I Subunit NDUFS1 Augment ROS Production following the Oxidation of Ubiquinone Linked Substrates, Glycerol-3-Phosphate and Proline.  |  Wang, K., et al. 2022. Antioxidants (Basel). 11: PMID: 36290766

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NDUFS1 Antibody (E-8) has 20 citations in a variety of scientific publications.

Primary antibodies like Anti-NDUFS1 Antibody (E-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NDUFS1 Antibody (E-8) Specifications

Alternate Product Name: NDUFS1

Product Clone Name: E-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 428-727

Epitope Target: NDUFS1

Epitope Species: human

Product Reactivity/Detection: NDUFS1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 20 citations in various scientific publications

Available Conjugates/Forms for NDUFS1 Antibody (E-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin