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NDH II Antibody (E-10): sc-137183

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Datasheets
  • NDH II Antibody (E-10) is a mouse monoclonal IgG1 κ, cited in 2 publications, provided at 200 µg/ml
  • raised against amino acids 1-300 mapping at the N-terminus of NDH II of human origin
  • recommended for detection of nuclear DNA helicase II of human origin by WB, IP, IF and ELISA
  • TransCruz reagent for ChIP application (sc-137183 X, 200 µg/0.1 ml)
  • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for NDH II Antibody (E-10) for WB applications. These reagents are now offered in bundles with NDH II Antibody (E-10) (see ordering information below).
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NDH II Antibody (E-10) is a mouse monoclonal IgG1 antibody that detects NDH II in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NDH II, also known as RNA helicase A, plays a pivotal role in the unwinding of RNA secondary structures, which is essential for various RNA processing events, including splicing and translation. Anti-NDH II antibody (E-10) facilitates the interaction of RNA-binding proteins with their target RNA molecules, thereby influencing gene expression and cellular responses. The proper functioning of NDH II is crucial for maintaining the integrity of RNA metabolism, and dysregulation of this helicase has been implicated in several diseases, including cancer. NDH II (E-10) antibody enables researchers to gain insights into the dynamics of RNA processing and the molecular mechanisms underlying various pathologies, making NDH II monoclonal antibody (E-10) an invaluable tool for studies in molecular biology and disease research.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

NDH II Antibody (E-10) References:

  1. Pre-mRNA and mRNA binding of human nuclear DNA helicase II (RNA helicase A).  |  Zhang, S., et al. 1999. J Cell Sci. 112 (Pt 7): 1055-64. PMID: 10198287
  2. Nucleolar localization of murine nuclear DNA helicase II (RNA helicase A).  |  Zhang, S., et al. 1999. J Cell Sci. 112 (Pt 16): 2693-703. PMID: 10413677
  3. Nuclear DNA helicase II (RNA helicase A) binds to an F-actin containing shell that surrounds the nucleolus.  |  Zhang, S., et al. 2004. Exp Cell Res. 293: 248-58. PMID: 14729462
  4. Werner syndrome helicase (WRN), nuclear DNA helicase II (NDH II) and histone gammaH2AX are localized to the centrosome.  |  Zhang, S., et al. 2007. Cell Biol Int. 31: 1109-21. PMID: 17498979
  5. Characterization of Nda2, a plastoquinone-reducing type II NAD(P)H dehydrogenase in chlamydomonas chloroplasts.  |  Desplats, C., et al. 2009. J Biol Chem. 284: 4148-57. PMID: 19056727
  6. Biochemical characterization of the cell-biomaterial interface by quantitative proteomics.  |  Tong, WY., et al. 2010. Mol Cell Proteomics. 9: 2089-98. PMID: 20562470
  7. Identification of DHX9 as a cell cycle regulated nucleolar recruitment factor for CIZ1.  |  Thacker, U., et al. 2020. Sci Rep. 10: 18103. PMID: 33093612
  8. Dynamically probing ATP-dependent RNA helicase A-assisted RNA structure conversion using single molecule fluorescence resonance energy transfer.  |  Wu, C., et al. 2021. Protein Sci. 30: 1157-1168. PMID: 33837988
  9. RNA Helicase A/DHX9 Forms Unique Cytoplasmic Antiviral Granules That Restrict Oncolytic Myxoma Virus Replication in Human Cancer Cells.  |  Rahman, MM., et al. 2021. J Virol. 95: e0015121. PMID: 33952639
  10. Molecular cloning of the gene encoding nuclear DNA helicase II. A bovine homologue of human RNA helicase A and Drosophila Mle protein.  |  Zhang, S., et al. 1995. J Biol Chem. 270: 16422-7. PMID: 7608213
  11. Identification of the product of ndhA gene as a thylakoid protein synthesized in response to photooxidative treatment.  |  Martín, M., et al. 1996. Plant Cell Physiol. 37: 293-8. PMID: 8673340
  12. Identification of DNA replication and cell cycle proteins that interact with PCNA.  |  Loor, G., et al. 1997. Nucleic Acids Res. 25: 5041-6. PMID: 9396813

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

NDH II Antibody (E-10)

sc-137183
200 µg/ml
$322.00

NDH II Antibody (E-10): m-IgG Fc BP-HRP Bundle

sc-537415
200 µg Ab; 10 µg BP
$361.00

NDH II Antibody (E-10): m-IgGκ BP-HRP Bundle

sc-534671
200 µg Ab; 40 µg BP
$361.00

NDH II Antibody (E-10) X

sc-137183 X
200 µg/0.1 ml
$322.00

When should the TransCruz reagent sc-137183 X be used instead of the standard form sc-137183?

Asked by: TinTin
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Exceptional immunofluorescence nuclear stainingExceptional immunofluorescence nuclear staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-01-07
Rated 5 out of 5 by from Produced positive Western Blot data of NDHProduced positive Western Blot data of NDH II expression in HeLa nuclear extract. -SCBT QC
Date published: 2013-01-20
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NDH II Antibody (E-10) is rated 5.0 out of 5 by 2.
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