NDH II Antibody (B-9): sc-137232

NDH II Antibody (B-9) is a mouse monoclonal IgG2b kappa light chain antibody that detects NDH II protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). NDH II (B-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NDH II protein, also known as nuclear DNA helicase II or RNA helicase A, plays a crucial role in regulating pre-mRNA splicing, a vital process in gene expression. NDH II protein is primarily located in the nucleus, where NDH II interacts with RNA-binding proteins to facilitate unwinding of RNA secondary structures, thereby promoting spliceosome assembly. This interaction ensures proper processing and transport of pre-mRNA, allowing only correctly spliced mRNA to be exported to the cytoplasm for translation. NDH II protein′s functionality is highlighted by involvement in forming large RNA-protein complexes, which are critical for mRNA maturation and stability. NDH II protein has been implicated in regulating various cellular processes, including cell growth and differentiation, particularly in human melanoma cells, where NDH II interacts with MDA5, an ATP-dependent RNA helicase. anti-NDH II antibody (B-9) versatility in various applications makes NDH II (B-9) monoclonal antibody an invaluable tool for researchers studying gene expression and RNA processing mechanisms.

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NDH II Antibody (B-9) References:

1. A novel shuttle protein binds to RNA helicase A and activates the retroviral constitutive transport element.  |  Westberg, C., et al. 2000. J Biol Chem. 275: 21396-401. PMID: 10748171
2. mda-5: An interferon-inducible putative RNA helicase with double-stranded RNA-dependent ATPase activity and melanoma growth-suppressive properties.  |  Kang, DC., et al. 2002. Proc Natl Acad Sci U S A. 99: 637-42. PMID: 11805321
3. RNA helicase A interacts with dsDNA and topoisomerase IIalpha.  |  Zhou, K., et al. 2003. Nucleic Acids Res. 31: 2253-60. PMID: 12711669
4. DNA-dependent protein kinase (DNA-PK) phosphorylates nuclear DNA helicase II/RNA helicase A and hnRNP proteins in an RNA-dependent manner.  |  Zhang, S., et al. 2004. Nucleic Acids Res. 32: 1-10. PMID: 14704337
5. Nuclear DNA helicase II (RNA helicase A) binds to an F-actin containing shell that surrounds the nucleolus.  |  Zhang, S., et al. 2004. Exp Cell Res. 293: 248-58. PMID: 14729462
6. Multiple functions of nuclear DNA helicase II (RNA helicase A) in nucleic acid metabolism.  |  Zhang, S. and Grosse, F. 2004. Acta Biochim Biophys Sin (Shanghai). 36: 177-83. PMID: 15202501
7. RNA helicase A interacts with nuclear factor kappaB p65 and functions as a transcriptional coactivator.  |  Tetsuka, T., et al. 2004. Eur J Biochem. 271: 3741-51. PMID: 15355351
8. RNA helicase A interacts with RISC in human cells and functions in RISC loading.  |  Robb, GB. and Rana, TM. 2007. Mol Cell. 26: 523-37. PMID: 17531811
9. RNA helicase A is a DNA-binding partner for EGFR-mediated transcriptional activation in the nucleus.  |  Huo, L., et al. 2010. Proc Natl Acad Sci U S A. 107: 16125-30. PMID: 20802156
10. A novel role of RNA helicase A in regulation of translation of type I collagen mRNAs.  |  Manojlovic, Z. and Stefanovic, B. 2012. RNA. 18: 321-34. PMID: 22190748
11. RNA helicase A is a downstream mediator of KIF1Bβ tumor-suppressor function in neuroblastoma.  |  Chen, ZX., et al. 2014. Cancer Discov. 4: 434-51. PMID: 24469107
12. Virion-associated, host-derived DHX9/RNA helicase A enhances the processivity of HIV-1 reverse transcriptase on genomic RNA.  |  Brady, S., et al. 2019. J Biol Chem. 294: 11473-11485. PMID: 31175158

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NDH II Antibody (B-9) has 15 citations in a variety of scientific publications.

Primary antibodies like Anti-NDH II Antibody (B-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NDH II Antibody (B-9) Specifications

Alternate Product Name: NDH II

Product Clone Name: B-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-300

Epitope Target: NDH II

Epitope Species: human

Product Reactivity/Detection: nuclear DNA helicase II

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 15 citations in various scientific publications

Available Conjugates/Forms for NDH II Antibody (B-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent