NCAM-L1 Antibody (D-5): sc-374046

NCAM-L1 Antibody (D-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects NCAM-L1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-NCAM-L1 antibody (D-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NCAM-L1, also known as L1 cell adhesion molecule or CD171, is a crucial member of the cell adhesion molecule family, which plays a significant role in mediating cell-cell interactions during development and growth. NCAM-L1 is involved in axon guidance and cell migration within the nervous system, making this protein essential for proper neuronal development and function. NCAM-L1 expression is particularly relevant in various human tumors, such as neuroblastomas, rhabdomyosarcomas, Wilms′ tumors, Ewing′s sarcomas, and certain primitive myeloid malignancies, where NCAM-L1 may facilitate tumor progression by enhancing cell adhesion and migration. NCAM-L1 presence in these contexts underscores this protein′s potential as a biomarker for malignancies and a target for therapeutic strategies aimed at disrupting tumor biology functions.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

NCAM-L1 Antibody (D-5) References:

1. Adhesion molecules CD171 (L1CAM) and CD24 are expressed by primary neuroendocrine carcinomas of the skin (Merkel cell carcinomas).  |  Deichmann, M., et al. 2003. J Cutan Pathol. 30: 363-8. PMID: 12834484
2. Reduced GABAergic transmission and number of hippocampal perisomatic inhibitory synapses in juvenile mice deficient in the neural cell adhesion molecule L1.  |  Saghatelyan, AK., et al. 2004. Mol Cell Neurosci. 26: 191-203. PMID: 15121190
3. An alcohol binding site on the neural cell adhesion molecule L1.  |  Arevalo, E., et al. 2008. Proc Natl Acad Sci U S A. 105: 371-5. PMID: 18165316
4. Identification of neural cell adhesion molecule L1-derived neuritogenic ligands of the fibroblast growth factor receptor.  |  Kulahin, N., et al. 2009. J Neurosci Res. 87: 1806-12. PMID: 19185025
5. Identification of cDNA clones of the mouse neural cell adhesion molecule L1.  |  Tacke, R., et al. 1987. Neurosci Lett. 82: 89-94. PMID: 2447532
6. CD171- and GD2-specific CAR-T cells potently target retinoblastoma cells in preclinical in vitro testing.  |  Andersch, L., et al. 2019. BMC Cancer. 19: 895. PMID: 31500597
7. Tumor-Derived Extracellular Vesicles Impair CD171-Specific CD4+ CAR T Cell Efficacy.  |  Ali, S., et al. 2020. Front Immunol. 11: 531. PMID: 32296437
8. Several extracellular domains of the neural cell adhesion molecule L1 are involved in neurite outgrowth and cell body adhesion.  |  Appel, F., et al. 1993. J Neurosci. 13: 4764-75. PMID: 8229197
9. Reduction of hippocampal long-term potentiation in transgenic mice ectopically expressing the neural cell adhesion molecule L1 in astrocytes.  |  Lüthi, A., et al. 1996. J Neurosci Res. 46: 1-6. PMID: 8892099
10. A single immunoglobulin-like domain of the human neural cell adhesion molecule L1 supports adhesion by multiple vascular and platelet integrins.  |  Felding-Habermann, B., et al. 1997. J Cell Biol. 139: 1567-81. PMID: 9396761

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NCAM-L1 Antibody (D-5) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-NCAM-L1 Antibody (D-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NCAM-L1 Antibody (D-5) Specifications

Alternate Product Name: L1CAM

Product Clone Name: D-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 921-1120

Epitope Target: NCAM-L1

Epitope Species: human

Product Reactivity/Detection: NCAM-L1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for NCAM-L1 Antibody (D-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred NCAM-L1 antibody is NCAM-L1 Antibody (UJ127.11): sc-53386.