Date published: 2025-11-22

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NCAM-L1 Antibody (5G3): sc-33686

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Datasheets
  • NCAM-L1 Antibody (5G3) is a mouse monoclonal IgG2a κ, cited in 7 publications, provided at 200 µg/ml
  • raised against human neuroblastoma cell line SK-N-AS
  • NCAM-L1 Antibody (5G3) is recommended for detection of NCAM-L1 of human origin by WB, IP, IF and FCM
  • Anti-NCAM-L1 Antibody (5G3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgG Fc BP-HRP is the preferred secondary detection reagent for NCAM-L1 Antibody (5G3) for WB applications. This reagent is now offered in a bundle with NCAM-L1 Antibody (5G3) (see ordering information below).

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SEE ALSO...

NCAM-L1 Antibody (5G3) is a mouse monoclonal IgG2a kappa light chain antibody that detects NCAM-L1 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). Anti-NCAM-L1 antibody (5G3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The neuronal cell adhesion molecule NCAM-L1, also known as CD171, is crucial for axon guidance and cell migration within the nervous system, playing a significant role in the development and maintenance of neural connections. NCAM-L1 expression is not only vital during embryogenesis but also has implications in various human tumors, such as neuroblastomas and rhabdomyosarcomas, where NCAM-L1 may facilitate tumor progression by enhancing cell adhesion and migration. NCAM-L1 presence in these malignancies underscores NCAM-L1′s potential as a biomarker for cancer progression and a target for therapeutic intervention. Anti-NCAM-L1 antibody (5G3) enables researchers to gain insights into the molecular mechanisms underlying neural development and tumor biology, making this valuable tool essential for studies in both basic and translational research.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

NCAM-L1 Antibody (5G3)

sc-33686
200 µg/ml
$316.00

NCAM-L1 Antibody (5G3): m-IgG Fc BP-HRP Bundle

sc-525543
200 µg Ab; 10 µg BP
$354.00

NCAM-L1 Antibody (5G3) AC

sc-33686 AC
500 µg/ml, 25% agarose
$416.00

NCAM-L1 Antibody (5G3) HRP

sc-33686 HRP
200 µg/ml
$316.00

NCAM-L1 Antibody (5G3) FITC

sc-33686 FITC
200 µg/ml
$330.00

NCAM-L1 Antibody (5G3) PE

sc-33686 PE
200 µg/ml
$343.00

NCAM-L1 Antibody (5G3) Alexa Fluor® 488

sc-33686 AF488
200 µg/ml
$357.00

NCAM-L1 Antibody (5G3) Alexa Fluor® 546

sc-33686 AF546
200 µg/ml
$357.00

NCAM-L1 Antibody (5G3) Alexa Fluor® 594

sc-33686 AF594
200 µg/ml
$357.00

NCAM-L1 Antibody (5G3) Alexa Fluor® 647

sc-33686 AF647
200 µg/ml
$357.00

NCAM-L1 Antibody (5G3) Alexa Fluor® 680

sc-33686 AF680
200 µg/ml
$357.00

NCAM-L1 Antibody (5G3) Alexa Fluor® 790

sc-33686 AF790
200 µg/ml
$357.00

Do you have by any chance this antibody conjugated to biotin?

Asked by: Maja
Thank you for your question. To request a biotin conjugated antibody please email specialorders@scbt.com for a price quote and ship date.
Answered by: BlakeJ
Date published: 2025-03-07

I would like to use NCAM-L1 (5G3): sc-33686 for IP applications. Does this antibody come pre-conjugated to agarose?

Asked by: Trav11
Thank you for your question. Please send an email request to specialorders@scbt.com for primary antibody conjugation not listed on our website. Also, you may want to consider using NCAM-L1 (D-5): sc-374046 as it is currently available conjugated to agarose, HRP, FITC, PE, Alexa Fluor 488, and Alexa Fluor 647. If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support 6
Date published: 2017-01-11

What is the recommended starting dilution for Western blot for NCAM-L1 (5G3): sc-33686?

Asked by: Trav11
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-33686.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support 6
Date published: 2017-01-11
  • y_2025, m_11, d_21, h_6CST
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Rated 5 out of 5 by from Nice FCM and WB !!Good antibody with strong western blot and FCM results
Date published: 2017-01-14
Rated 5 out of 5 by from FACS/FCMGreat clone for use in flow cytometry with a fluorescent secondary.
Date published: 2016-12-23
Rated 4 out of 5 by from SCSC-33686 I would rate a 4 because UJ127.11 works better in a comparison, but it works great as a capture antibody in ELISA.
Date published: 2015-08-18
Rated 4 out of 5 by from Nice band achieved in WB with IMRNice band achieved in WB with IMR-32 whole cell lysate. -SCBT QC
Date published: 2015-03-28
Rated 5 out of 5 by from Publishable WB data obtained breast cancerPublishable WB data obtained breast cancer cell lines. -SCBT Publication Review
Date published: 2015-02-14
Rated 5 out of 5 by from Superb indirect FCM data of IMRSuperb indirect FCM data of IMR-32 cells stained with NCAM-L1 . -SCBT QC
Date published: 2014-11-03
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NCAM-L1 Antibody (5G3) is rated 4.7 out of 5 by 6.
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