NASP Antibody (A-7): sc-514669
- NASP Antibody (A-7) is a mouse monoclonal IgG2a κ NASP antibody, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 550-572 within an internal region of NASP of human origin
- NASP Antibody (A-7) is recommended for detection of NASP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-NASP Antibody (A-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for NASP Antibody (A-7) for WB and IHC(P) applications. These reagents are now offered in bundles with NASP Antibody (A-7) (see ordering information below).
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NASP Antibody (A-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects NASP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). NASP (A-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NASP, or nuclear autoantigenic sperm protein, is a crucial player in the transport of histones to the nucleus, which is essential for the assembly of nucleosomes and the regulation of gene expression. This 788 amino acid protein is localized in both the nucleus and cytoplasm and features three tetratricopeptide repeats, which are important for protein-protein interactions. NASP is expressed in multiple alternatively spliced isoforms, including a testis- and sperm-specific variant (tNASP) and a somatic cell variant (sNASP). NASP functions as a Histone H1 binding protein to facilitate the transport of histones necessary for proper nucleosome formation, thereby ensuring normal cell cycle progression and cellular proliferation. NASP plays a vital role in spermatogenesis and normal development due to significant involvement in DNA replication and cell cycle regulation. In response to DNA damage, NASP undergoes phosphorylation by ATM or ATR, highlighting involvement in cellular stress responses.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
NASP Antibody (A-7) References:
- Characterization of the histone H1-binding protein, NASP, as a cell cycle-regulated somatic protein. | Richardson, RT., et al. 2000. J Biol Chem. 275: 30378-86. PMID: 10893414
- Analysis of the autoimmune epitopes on human testicular NASP using recombinant and synthetic peptides. | Batova, IN., et al. 2000. Clin Exp Immunol. 121: 201-9. PMID: 10931132
- Analysis of gene expression in mouse 2-cell embryos using fluorescein differential display: comparison of culture environments. | Minami, N., et al. 2001. Biol Reprod. 64: 30-5. PMID: 11133655
- Comparison of mouse and human NASP genes and expression in human transformed and tumor cell lines. | Richardson, RT., et al. 2001. Gene. 274: 67-75. PMID: 11674998
- Overexpression of the Linker histone-binding protein tNASP affects progression through the cell cycle. | Alekseev, OM., et al. 2003. J Biol Chem. 278: 8846-52. PMID: 12509435
- Nuclear autoantigenic sperm protein (NASP), a linker histone chaperone that is required for cell proliferation. | Richardson, RT., et al. 2006. J Biol Chem. 281: 21526-21534. PMID: 16728391
- sNASP, a histone H1-specific eukaryotic chaperone dimer that facilitates chromatin assembly. | Finn, RM., et al. 2008. Biophys J. 95: 1314-25. PMID: 18456819
- Expanded binding specificity of the human histone chaperone NASP. | Wang, H., et al. 2008. Nucleic Acids Res. 36: 5763-72. PMID: 18782834
- Histone-binding domains in a human nuclear autoantigenic sperm protein. | Batova, I. and O'Rand, MG. 1996. Biol Reprod. 54: 1238-44. PMID: 8724350
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NASP Antibody (A-7) | sc-514669 | 200 µg/ml | $322.00 | |||
NASP Antibody (A-7): m-IgG Fc BP-HRP Bundle | sc-531187 | 200 µg Ab; 10 µg BP | $361.00 | |||
NASP Antibody (A-7): m-IgGκ BP-HRP Bundle | sc-524806 | 200 µg Ab, 40 µg BP | $361.00 | |||
NASP Antibody (A-7): m-IgG2a BP-HRP Bundle | sc-547718 | 200 µg Ab; 10 µg BP | $361.00 | |||
NASP Antibody (A-7) AC | sc-514669 AC | 500 µg/ml, 25% agarose | $424.00 | |||
NASP Antibody (A-7) HRP | sc-514669 HRP | 200 µg/ml | $322.00 | |||
NASP Antibody (A-7) FITC | sc-514669 FITC | 200 µg/ml | $336.00 | |||
NASP Antibody (A-7) PE | sc-514669 PE | 200 µg/ml | $349.00 | |||
NASP Antibody (A-7) Alexa Fluor® 488 | sc-514669 AF488 | 200 µg/ml | $364.00 | |||
NASP Antibody (A-7) Alexa Fluor® 546 | sc-514669 AF546 | 200 µg/ml | $364.00 | |||
NASP Antibody (A-7) Alexa Fluor® 594 | sc-514669 AF594 | 200 µg/ml | $364.00 | |||
NASP Antibody (A-7) Alexa Fluor® 647 | sc-514669 AF647 | 200 µg/ml | $364.00 | |||
NASP Antibody (A-7) Alexa Fluor® 680 | sc-514669 AF680 | 200 µg/ml | $364.00 | |||
NASP Antibody (A-7) Alexa Fluor® 790 | sc-514669 AF790 | 200 µg/ml | $364.00 | |||
NASP (A-7) Neutralizing Peptide | sc-514669 P | 100 µg/0.5 ml | $69.00 |