Nanog Antibody (H-2): sc-374103

Nanog Antibody (H-2) is a mouse monoclonal IgG2a kappa light chain antibody engineered to detect Nanog from mouse, rat, and human origins through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Nanog monoclonal antibody (H-2) is raised against amino acids 151-305 at the C-terminus of human Nanog, ensuring precise recognition and binding to the targeted epitope. Nanog, also called Nanog homeobox, is a critical homeodomain-containing transcription factor that plays an essential role in maintaining pluripotency and self-renewal of embryonic stem cells, thereby preventing differentiation. Structurally, Nanog comprises a conserved homeodomain that facilitates specific DNA binding, as well as regions that enable interactions with other key proteins involved in the pluripotency network, such as Oct4 and Sox2. These interactions are vital for forming transcriptional complexes that regulate the expression of genes necessary for sustaining the undifferentiated state of stem cells. Additionally, Nanog interacts with proteins like KLF4 and TBX3, which further contribute to the regulation of stem cell maintenance and inhibition of differentiation pathways. The ability of Nanog to form complexes with these proteins underscores a pivotal role in orchestrating gene expression programs that maintain cellular pluripotency and drive self-renewal. By targeting these interaction domains, anti-Nanog antibody (H-2) allows researchers to investigate the molecular mechanisms by which Nanog collaborates with binding partners to control stem cell fate decisions. This makes Nanog (H-2) antibody an indispensable tool for studies in stem cell biology, developmental biology, and regenerative medicine, facilitating advancements in understanding how pluripotent states are maintained and how differentiation can be precisely controlled.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Nanog Antibody (H-2) has 27 citations in a variety of scientific publications.

Primary antibodies like Anti-Nanog Antibody (H-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Nanog Antibody (H-2) Specifications

Alternate Product Name: Nanog homeobox

Product Clone Name: H-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 151-305

Epitope Target: Nanog

Epitope Species: human

Product Reactivity/Detection: Nanog

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 27 citations in various scientific publications

Available Conjugates/Forms for Nanog Antibody (H-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Nanog antibody is Nanog Antibody (5A10): sc-134218.