Nanog Antibody (5A10) recommended for detection of Nanog of mouse, rat and human origin by WB, IP and ELISA

Nanog Antibody (5A10): sc-134218

Nanog Antibody (5A10) is rated 4.9 out of 5 by 7.
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Datasheets

    • Nanog Antibody (5A10) is a mouse monoclonal IgG2a, cited in 23 publications, provided at 50 µg/500 µl
    • raised against amino acids 1-154 corresponding to recombinant Nanog of human origin
    • recommended for detection of Nanog of mouse, rat and human origin by WB, IP and ELISA
    • See Nanog (1E6C4): sc-293121 for Nanog antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Nanog (5A10): sc-134218.

Is this Nanog (5A10): sc-134218 monoclonal antibody available directly conjugated to HRP?

Asked by: cjMara
Thank you for your question. All requests for directly conjugated versions of this antibody should be directed to our Special Orders team at [email protected] Alternatively, consider using Nanog (1E6C4): sc-293121, which is available conjugated to agarose, HRP, FITC, PE, and more. (https://www.scbt.com/scbt/product/nanog-antibody-1e6c4)
Answered by: Technical Support
Date published: 2017-01-30
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Rated 4 out of 5 by from Buon funzionamento Ho provato l' anticorpo primario Nanog (5A10):sc-134218. Lo ho usato per western blot e devo dire che il risultato è stato soddisfacente. Ho usato una concentrazione (1:2000) più bassa di quella suggerita (1:1000) ed ho avuto un buon risultato lo stesso su due membrane su cui avevo corso 30µ e 40 µg di proteine. L’incubazione con il primario è stata fatta ON a 4°C ed ho usato un secondario anti mouse 1:20000. Le bande sono nette e c’è poco aspecifico. Dopo l’incubazione con primario e dopo l’incubazione con secondario ho eseguito 3x10 min di lavaggi con TPBS (PBS+0.1% Tween) ed il blocking è stato fatto per 4 ore con Milk al 5% in TPBS a temperatura ambiente. Ritengo sia un prodotto valido per il mio tipo di ricerca e per il western blot in generale.
Date published: 2020-07-21
Rated 5 out of 5 by from very pleased That was a very good antibody, diluted 1:1000 in human ovary tissue , we obtained good results.
Date published: 2017-05-11
Rated 5 out of 5 by from great Clean bands obtained in western blot using human ovary tissue extract.
Date published: 2017-04-17
Rated 5 out of 5 by from Produced publishable WB data on human and mouse Well characterized with publications in the primary literature for WB in human and mouse samples
Date published: 2017-01-11
Rated 5 out of 5 by from WB: strong WB: strong, clean band in NIH/3T3 whole cell lysate. -SCBT QC
Date published: 2015-03-22
Rated 5 out of 5 by from Positive results in WB with transfected lysates Positive results in WB with transfected lysates. -SCBT QC
Date published: 2015-02-03
Rated 5 out of 5 by from Clean bands in western blot analysis of Nanog Clean bands in western blot analysis of Nanog expression in HeLa and Raji whole cell lysates and human ovary tissue extract. -SCBT QC
Date published: 2013-08-25
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