Nanog Antibody (1E6C4): sc-293121

Nanog Antibody (1E6C4) is a mouse monoclonal IgG1 kappa light chain antibody designed to detect human Nanog protein with precision across applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Nanog antibody (1E6C4) is raised against amino acids 20-166 of the recombinant human Nanog protein, ensuring robust recognition and binding to the target epitope. Nanog, also called Nanog homeobox, is a pivotal homeodomain-containing transcription factor that plays a crucial role in maintaining pluripotency and self-renewal of embryonic stem cells. Structurally, Nanog comprises a conserved homeodomain that facilitates specific DNA binding, enabling regulation of genes essential for sustaining the undifferentiated state of stem cells. Additionally, Nanog contains regions outside the homeodomain involved in protein-protein interactions, which are vital for forming complexes that modulate gene expression networks. The structural integrity of Nanog is fundamental to orchestrating the delicate balance between stem cell maintenance and differentiation. By targeting these critical structural domains, Nanog monoclonal antibody (1E6C4) allows researchers to accurately investigate the molecular mechanisms by which Nanog governs pluripotency and directs cellular fate decisions. Anti-Nanog antibody (1E6C4) serves as an indispensable tool in stem cell biology, developmental biology, and regenerative medicine, facilitating advancements in understanding how stem cells can be manipulated for therapeutic purposes. With high affinity and specificity for human Nanog protein, Nanog (1E6C4) antibody supports comprehensive studies aimed at unraveling the complexities of stem cell regulation and the potential for harnessing pluripotent cells in various biomedical applications.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Nanog Antibody (1E6C4) has 151 citations in a variety of scientific publications.

Primary antibodies like Anti-Nanog Antibody (1E6C4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Nanog Antibody (1E6C4) Specifications

Alternate Product Name: Nanog homeobox

Product Clone Name: 1E6C4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 20-166

Epitope Target: Nanog

Epitope Species: human

Product Reactivity/Detection: Nanog

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 151 citations in various scientific publications

Available Conjugates/Forms for Nanog Antibody (1E6C4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Nanog antibody is Nanog Antibody (5A10): sc-134218.