Anti-Nanog Antibody (1E6C4) recommended for detection of Nanog of human origin by WB, IP, IF and ELISA

Anti-Nanog Antibody (1E6C4): sc-293121

Anti-Nanog Antibody (1E6C4) is rated 3.9 out of 5 by 29.
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Datasheets

    • Nanog Antibody (1E6C4) is a mouse monoclonal IgG1 κ, cited in 108 publications, provided at 200 µg/ml
    • raised against amino acids 20-166 corresponding to recombinant Nanog of human origin
    • Nanog Antibody (1E6C4) is recommended for detection of Nanog of human origin by WB, IP, IF and ELISA
    • Anti-Nanog Antibody (1E6C4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Nanog (1E6C4): sc-293121.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Nanog Antibody (1E6C4) for WB applications. These reagents are now offered in bundles with Nanog Antibody (1E6C4) (see ordering information below).
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Has this product been discontinued? Because our nearest distributor is saying so. We are very much interested for this Antibody along with other two. Please do reply.

Asked by: Dr D Jena
Thank you for your question. This antibody is available and in stock. If your distributor is unable to get this to you, please contact an alternate distributor.
Answered by: Tech Service
Date published: 2019-01-07

I would like to use sc-293121 for some double staining. Any tips for using only mouse monoclonal antibodies?

Asked by: Cweed
We recommend using directly conjugated antibodies. This product, sc-293121, is available conjugated to FITC, AF488, and AF647.
Answered by: Technical Support 8
Date published: 2017-01-20
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Rated 1 out of 5 by from Does not work well on hiPS cells with very simple procedure 4% PFA, 15 min, RT fixation hiPS cells 1:50 dilution 4℃, O/N incubation
Date published: 2021-08-05
Rated 1 out of 5 by from resullt i don't know why the whole-cell bright,not noly uncleus.....
Date published: 2019-07-27
Rated 4 out of 5 by from Pluripotency factor immunofluorescence This antibody worked well for pluripotency factor detection by immunofluorescence.
Date published: 2018-09-25
Rated 5 out of 5 by from staining for feeder free iPSCs It works well for feeder-free iPSCs. We have got images with little background noise.
Date published: 2018-07-11
Rated 3 out of 5 by from Ok Western Blot, Mauvais en IF J'ai testé cette en anticorps en IF mais je n'ai eu aucun signal (1/50e ; 1/100e ; 1/200) avec un démasquage à pH 6 et pH9. En revanche, il fonctionne en Western Blot, dilution 1/500e
Date published: 2018-06-07
Rated 4 out of 5 by from ottimo funzionamento L'anticorpo è davvero ottimo! banda netta ll'altezza di 50K
Date published: 2018-04-04
Rated 1 out of 5 by from did not wok on mouse tissue IHC(P) We tried this antibody on paraffin sections of paraformaldehyde fixed mouse embryonic gonads and could not detect any signal.
Date published: 2018-03-20
Rated 4 out of 5 by from Good Good it picks single band in many of the cancer cell lines at 1:200, but with little less affinity seems.
Date published: 2018-02-28
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