Nanog Antibody (5A10): sc-134218

Nanog Antibody (5A10) is a mouse monoclonal IgG2a antibody designed to detect Nanog across mouse, rat, and human species through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-Nanog antibody (5A10) targets amino acids 1-154 of the recombinant human Nanog protein, ensuring precise recognition and binding. Nanog, also called Nanog homeobox, is a critical homeodomain transcription factor localized predominantly in the nucleus of undifferentiated embryonic stem cells, where Nanog plays an essential role in maintaining pluripotency and regulating the balance between self-renewal and differentiation. The nuclear localization of Nanog is vital for orchestrating gene expression programs that sustain the undifferentiated state of stem cells, making Nanog a key factor in developmental biology and regenerative medicine research. By facilitating accurate detection and analysis of Nanog, anti-Nanog antibody (5A10) enables researchers to investigate molecular mechanisms underlying stem cell pluripotency, cellular reprogramming, and differentiation processes. Nanog monoclonal antibody (5A10) serves as an invaluable tool for advancing studies in stem cell biology, developmental biology, and therapeutic strategies aimed at tissue regeneration and repair. With robust reactivity and high affinity for Nanog, Nanog (5A10) antibody supports comprehensive exploration of Nanog′s role in maintaining the undifferentiated state of cells, thereby contributing to significant advancements in understanding and manipulating cellular fate.

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Nanog Antibody (5A10) References:

1. Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells.  |  Chambers, I., et al. 2003. Cell. 113: 643-55. PMID: 12787505
2. Identification of two distinct transactivation domains in the pluripotency sustaining factor nanog.  |  Pan, GJ. and Pei, DQ. 2003. Cell Res. 13: 499-502. PMID: 14728807
3. Human STELLAR, NANOG, and GDF3 genes are expressed in pluripotent cells and map to chromosome 12p13, a hotspot for teratocarcinoma.  |  Clark, AT., et al. 2004. Stem Cells. 22: 169-79. PMID: 14990856
4. Identification, cloning and expression analysis of the pluripotency promoting Nanog genes in mouse and human.  |  Hart, AH., et al. 2004. Dev Dyn. 230: 187-98. PMID: 15108323
5. Eleven daughters of NANOG.  |  Booth, HA. and Holland, PW. 2004. Genomics. 84: 229-38. PMID: 15233988
6. Sequential DNA methylation of the Nanog and Oct-4 upstream regions in human NT2 cells during neuronal differentiation.  |  Deb-Rinker, P., et al. 2005. J Biol Chem. 280: 6257-60. PMID: 15615706
7. Stem cell pluripotency factor NANOG is expressed in human fetal gonocytes, testicular carcinoma in situ and germ cell tumours.  |  Hoei-Hansen, CE., et al. 2005. Histopathology. 47: 48-56. PMID: 15982323

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Nanog Antibody (5A10) has 38 citations in a variety of scientific publications.

Primary antibodies like Anti-Nanog Antibody (5A10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Nanog Antibody (5A10) Specifications

Alternate Product Name: Nanog homeobox

Product Clone Name: 5A10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-154

Epitope Target: Nanog

Epitope Species: human

Product Reactivity/Detection: Nanog

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 50 µg IgG_2a kappa light chain in 500 µl of PBS with < 0.1% sodium azide, 0.1% gelatin and 1% glycerol

Research Citations: 38 citations in various scientific publications

Available Conjugates/Forms for Nanog Antibody (5A10): N/A